Identification of dinucleoside polyphosphates by matrix-assisted laser desorption/ionisation post-source decay mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Joachim JankowskiHartmut Schlüter

Abstract

Dinucleoside polyphosphates are a group of intra- and extracellular mediators controlling numerous physiological functions. In this study dinucleoside polyphosphates were examined by positive ion matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MADLI-TOFMS). 3-Hydroxypicolinic acid was used as UV-absorbing matrix. For the individual dinucleoside polyphosphates Ap(n)A (n = 2-7), Ap(n)G (n = 2-6) and Gp(n)G (n = 2-6), MALDI post-source decay (PSD) mass spectra were measured. Each mass peak in the MALDI-PSD mass spectra could be assigned to individual fragments of dinucleoside polyphosphates. The comparison of the fragmentation patterns of the dinucleoside polyphosphates presented here demonstrates that dinucleoside polyphosphates preferably cleave to fragment ions consisting of the corresponding mononucleoside polyphosphates as well as the corresponding nucleosides and bases during flight in the field-free drift path of the MALDI mass spectrometer. Therefore, the MALDI-PSD approach described here is suitable for identification of other dinucleoside polyphosphates. The present MALDI-PSD mass spectra may be used as MALDI-PSD mass reference spectra for future identification of dinucleoside polyphosphates...Continue Reading

References

Apr 24, 1987·Nucleic Acids Research·K E Ng, L E Orgel
Dec 30, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·J JankowskiH Schlüter
Aug 14, 1999·The Journal of Biological Chemistry·J JankowskiH Schlüter

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Citations

Feb 6, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·V JankowskiJ Jankowski
Mar 19, 2013·PloS One·Doreen JankeVera Jankowski
Aug 8, 2013·PloS One·Vera JankowskiJoachim Jankowski
Oct 13, 2006·Journal of Endocrinological Investigation·J LuoJ Jankowski
Sep 25, 2003·Journal of Mass Spectrometry : JMS
Jan 18, 2006·Chemistry : a European Journal·Federico PepiMarco Di Stefano
Nov 29, 2013·Mass Spectrometry Reviews·Ed Dudley, Liz Bond
May 30, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anna SchulzJoachim Jankowski

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