Identification of DNA-reactive B cells in patients with systemic lupus erythematosus.

Journal of Immunological Methods
Jie ZhangBetty Diamond

Abstract

Autoreactive B cells play a central role in systemic lupus erythematosus (SLE). Characterization of DNA-reactive B cells in the blood of lupus patients has been limited by the low frequency of the population. Using a tetrameric configuration of a peptide mimetope of DNA, we identified peptide-reactive B cells in peripheral blood. Antibodies derived from these B cells bound to peptide and were largely cross-reactive to dsDNA. This methodology enables us to track the development of autoreactive B cells, which recognize peptide and dsDNA, in individual patients with SLE and permits the isolation of autoreactive B cells for further characterization.

References

Jun 1, 1992·Kidney International·D V VlahakosM P Madaio
Sep 1, 1995·Kidney International·M R EhrensteinD A Isenberg
Dec 31, 2002·Journal of Immunological Methods·Jeffrey NewmanBetty Diamond
Aug 16, 2003·Science·Hedda WardemannMichel C Nussenzweig
Jan 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey S RiceBetty Diamond
Mar 2, 2005·The Journal of Experimental Medicine·Sergey YurasovMichel C Nussenzweig
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Patricio T HuertaBetty Diamond
Dec 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Czeslawa KowalBetty Diamond

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Citations

May 9, 2009·Nature Reviews. Immunology·Betty DiamondBruce T Volpe
Oct 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Thomas W FaustPatricio T Huerta
Apr 29, 2009·Journal of Autoimmunity·Jie ZhangBetty Diamond
Jul 10, 2013·Journal of Autoimmunity·Giovanni FranchinBetty Diamond
Nov 23, 2019·Immunological Reviews·Kevin S CashmanIñaki Sanz
Apr 6, 2017·Scientific Reports·Vincent GiesPauline Soulas-Sprauel

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