Identification of dominant sulfamethoxazole-degraders in pig farm-impacted soil by DNA and protein stable isotope probing

Environment International
Wei-Ying OuyangLorenz Adrian

Abstract

Increasing introduction of antibiotic residues from humans and animal farming into the environment impacts the functioning of natural ecosystems and significantly contributes to the propagation of antibiotic resistance. Microbial degradation is the major sink of antibiotics in soil but the identification of in situ degrading populations is challenging. Here, we investigated sulfamethoxazole-degrading bacteria in soil microcosms by culture-independent DNA and protein stable isotope probing. 0.5% of the carbon from 13C6-labeled sulfamethoxazole amended to soil microcosms was transformed to 13CO2 demonstrating partial mineralization of the antibiotic. DNA stable isotope probing revealed incorporation of 13C from 13C6-labeled sulfamethoxazole into Actinobacteria and among them into the families Intrasporangiaceae, Nocardioidaceae, and Gaiellaceae and the order Solirubrobacterales. Protein stable isotope probing demonstrated the incorporation of 13C from 13C6-labeled sulfamethoxazole into proteins of bacteria of the families Intrasporangiaceae, Nocardioidaceae and the order Solirubrobacterales, which is consistent with the results of DNA stable isotope probing. The 13C abundance of 60 to 80% in several taxonomically relevant protein...Continue Reading

Citations

Mar 21, 2020·International Journal of Environmental Research and Public Health·Xuanjiang YangQiang Zhang
Nov 12, 2020·Applied Microbiology and Biotechnology·Olga C NunesPhilippe F-X Corvini
Mar 15, 2021·Journal of Environmental Management·María Rosa Pino-OtínDiego Ballestero
Jun 13, 2021·Environmental Science and Pollution Research International·Sara GallegoFabrice Martin-Laurent
Jun 25, 2021·Clinical, Cosmetic and Investigational Dermatology·Zhifen QiaoQi Xiang
Aug 29, 2021·The Science of the Total Environment·Paula GuedesCristina Silva Pereira
Aug 31, 2021·Ecotoxicology and Environmental Safety·Fabienne ReißStefan Kalkhof

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