Identification of drug targets and potential molecular mechanisms for Wantong Jingu Tablet extract in treatment of rheumatoid arthritis: bioinformatics analysis of fibroblast-like synoviocytes

Chinese Medicine
Zhaodong LiFan Li

Abstract

Rheumatoid arthritis-fibroblast-like synoviocytes (RA-FLSs) play important roles in pathogenesis of rheumatoid arthritis (RA). Wantong Jingu Tablet (WJT), a mixture of traditional Chinese medicine, is a potentially effective therapy for RA, but its underlying mechanism is unclear. In this study, we explore the effects of WJT on human RA-FLSs and the underlying molecular mechanism. The major components of WJT were determined using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS). Cell proliferative ability was evaluated by CCK-8, colony formation assay, and EdU incorporation assay. Cell apoptotic capacity was examined by caspase-3 and caspase-9 activity test. Protein levels of Bax and Bcl-2 were investigated by western blotting. High-throughput sequencing and bioinformatics analysis were conducted to screen and identify targeted genes, followed by identification by qRT-PCR and western blotting. In this study, we have identified 346 compounds in WJT. Our results showed that WJT inhibited the RA-FLSs proliferation, and promoted apoptosis in a dose- and time-dependent manner. More importantly, 184 differentially expressed genes (DEGs) has been screened after WJT t...Continue Reading

References

Sep 24, 2008·International Journal of Experimental Pathology·Shigeru TakahashiMinoru Wakita
Jan 8, 2015·Science Signaling·Shyam NyatiAlnawaz Rehemtulla
Mar 24, 2016·Molecular and Cellular Biology·Meenalakshmi ChinnamDavid W Goodrich
Mar 30, 2017·International Journal of Molecular Sciences·Yongchen GuoWancai Yang
Apr 30, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Chunping YaoJinming Yu
May 13, 2017·Seminars in Immunopathology·M Asif AminJeffrey H Ruth
Jun 2, 2017·Arthritis Research & Therapy·Marta F BustamanteMonica Guma
Oct 11, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jinxiang LiuJuan Du
Mar 5, 2018·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Lanni AquilaAnna Woloszynska-Read
Mar 27, 2018·Arthritis & Rheumatology·Jane FalconerChristopher D Buckley
Mar 28, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Weiqiong WangHuanhuan Quan
Sep 19, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yarigui BaoChun-Su Yuan
Mar 28, 2019·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Yuan XiongGuo-Hui Liu
Apr 28, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kun ChenQi Li
May 31, 2019·Journal of Integrative Bioinformatics·Boas PuckerSina Franziska Schumacher

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-seq
scanning electron microscopy
PCR
PCA

Software Mentioned

WGCNA
DESeq2 R
R package
STEM
edgeR
clusterProfiler R package
DESeq2
Cytoscape
Image J
SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis