Identification of Endogenous Site-specific Covalent Binding of Catechol Estrogens to Serum Proteins in Human Blood

Toxicological Sciences : an Official Journal of the Society of Toxicology
Chieh-Ming FangShu-Hui Chen

Abstract

Protein adducts covalently modified by catechol estrogens (CEs), referred to as estrogenized proteins, are potential biomarkers for estrogen homeostasis or exposure to environmental toxicants. However, serum proteins endogenously modified by CEs and the modification sites remain elusive. In this study, liquid chromatography-mass spectrometry (LC-MS)-based shotgun proteomics is applied to identify site-specific protein estrogenization in human blood via a systematic approach and stringent validation. We showed CEs, namely 2- and 4-hydroxyl estrogens which are regarded as biomarkers for estrogen homeostasis, form covalent bonds with proteins, mainly via side chain Cys, Lys, or His residue. Estrogenization of purified human serum albumin (HSA) and immunoglobulin G (IgG) at specific sites was achieved by co-incubation and used as the standards to confirm the identified estrogenization in serum proteins. Based on a database search, estrogenized peptides derived from serum proteins in patient blood were identified; endogenous estrogenization of HSA and IgG-1 at multiple sites were confirmed as compared to the standards. Based on a test using Ellman's reagent, estrogenization produced stable products and irreversibly abolished the rea...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S StamlerJ Loscalzo
Nov 2, 1983·European Journal of Biochemistry·K TakabayashiY Inada
Mar 1, 1996·Chemical Research in Toxicology·Y J Abul-HajjW H Ojala
Aug 30, 2000·Journal of the National Cancer Institute. Monographs·E CavalieriD Roy
Apr 11, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·J T SandersonM van den Berg
Jul 12, 2002·Analytical and Bioanalytical Chemistry·Christian K RienerHermann J Gruber
Nov 8, 2002·Proteomics·David M Creasy, John S Cottrell
Sep 20, 2005·Journal of Molecular Biology·Jamie GhumanStephen Curry
Dec 1, 2006·The Journal of Pharmacology and Experimental Therapeutics·Yu IshimaMasaki Otagiri
Dec 7, 2007·Chemical Research in Toxicology·Daniel C Liebler
May 14, 2008·FEBS Letters·Marjolaine RocheEmmanuel Bourdon
Oct 23, 2008·The Journal of Biological Chemistry·Yu IshimaMasaki Otagiri
Mar 5, 2009·Journal of Proteome Research·Michael R HoopmannMichael J MacCoss
Oct 3, 2009·Science Signaling·Tanja K Rudolph, Bruce A Freeman
Feb 27, 2013·Chemical Reviews·Yaoyang ZhangJohn R Yates
Oct 23, 2013·Chemical Research in Toxicology·Stefania NicolisLuigi Casella

❮ Previous
Next ❯

Citations

Jun 13, 2018·Journal of Clinical Biochemistry and Nutrition·Yoji Kato, Naoko Suga
May 1, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Qiaomei SunHui Li
Jun 26, 2020·International Immunopharmacology·Wahid Ali KhanMohd Wajid Ali Khan
May 15, 2020·Journal of Chemical Information and Modeling·Hao LiangLuhua Lai
Jul 22, 2020·Chemical Research in Toxicology·Catarina CharneiraAlexandra M M Antunes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.