Identification of endonuclease domain-containing 1 gene in Japanese flounder Paralichthys olivaceus

Fish & Shellfish Immunology
Zhe-Zhe LyuHidehiro Kondo

Abstract

The mRNA level of the endonuclease domain-containing 1 gene (Jf_ENDOD1) in Japanese flounder Paralichthys olivaceus kidney was significantly increased after injection of formalin-killed bacteria cells (FKC) in the previous microarray study. ENDOD1 is a member of the DNA/RNA non-specific nucleases family, and its role in fish immunity has not been reported. The open reading frame of Jf_ENDOD1 cDNA was 912 bp, encoding 303 amino acids. The first 27 amino acids were predicted to be a signal peptide and the mature Jf_ENDOD1 was calculated as 32 kDa. The amino acid sequence of Jf_ENDOD1 showed 76% identity to that of large yellow croaker Larimichthys crocea. Transcripts of Jf_ENDOD1 were marginally detected in all sampled tissues from healthy fish, while they were significantly detected in brain, kidney, spleen and intestine at 6 h post FKC injection. Jf_ENDOD1 recombinant protein produced in Escherichia coli showed DNase activity. Furthermore, to evaluate the DNase activities in vivo, total proteins from Japanese flounder kidney and spleen were extracted at 12, 24 and 72 h post Edwardsiella tarda FKC injection. The DNase activity of extracted protein was higher in treated fish than in untreated fish. Since the mRNA levels were sign...Continue Reading

References

Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J C BardwellJ Beckwith
Feb 8, 1997·Lancet·R E Hancock
Mar 6, 1998·Trends in Biotechnology·R E Hancock, R Lehrer
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·P H BessetteG Georgiou
Dec 7, 2000·The New England Journal of Medicine·J A Lekstrom-Himes, J I Gallin
Jan 25, 2002·Nature·Michael Zasloff
Dec 6, 2003·Genome Research·Gila Lithwick, Hanah Margalit
Mar 6, 2004·Science·Volker BrinkmannArturo Zychlinsky
Jul 9, 2004·Molecular & Cellular Proteomics : MCP·Qiang TianLeroy E Hood
Oct 2, 2004·Biotechnology Progress·Kouhei TsumotoTsutomu Arakawa
Jan 11, 2005·Current Protein & Peptide Science·Dawn M E BowdishRobert E W Hancock
May 14, 2005·Fish & Shellfish Immunology·J H W M RomboutG Scapigliati
Jun 14, 2005·Fish & Shellfish Immunology·Bergljót Magnadóttir
Jun 16, 2005·Current Opinion in Immunology·Joost J Oppenheim, De Yang
Aug 17, 2005·Nature Reviews. Molecular Cell Biology·Kumiko Samejima, William C Earnshaw
Dec 20, 2005·Cell·Ido GoldingEdward C Cox
May 19, 2009·Nature Medicine·Kai KessenbrockDieter E Jenne
Oct 24, 2009·FEBS Letters·Tobias MaierLuis Serrano
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Abdul HakkimArturo Zychlinsky
Mar 11, 2011·Science Translational Medicine·Gina S Garcia-RomoVirginia Pascual

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.