Identification of epithelial label-retaining cells at the transition between the anal canal and the rectum in mice.

Cell Cycle
Laura A RunckGeraldine Guasch

Abstract

In certain regions of the body, transition zones exist where stratified squamous epithelia directly abut against other types of epithelia. Certain transition zones are especially prone to tumorigenesis an example being the anorectal junction, although the reason for this is not known. One possibility is that the abrupt transition of the simple columnar epithelium of the colon to the stratified squamous epithelium of the proximal portion of the anal canal may contain a unique stem cell niche. We investigated whether the anorectal region contained cells with stem cell properties relative to the adjacent epithelium. We utilized a tetracycline-regulatable histone H2B-GFP transgenic mice model, previously used to identify hair follicle stem cells, to fluorescently label slow-cycling anal epithelial cells (e.g., prospective stem cells) in combination with a panel of putative stem cell markers. We identified a population of long-term GFP label-retaining cells concentrated at the junction between the anal canal and the rectum. These cells are BrdU-retaining cells and expressed the stem cell marker CD34. Moreover, tracking the fate of the anal label-retaining cells in vivo revealed that the slow-cycling cells only gave rise to progeny o...Continue Reading

References

May 1, 1986·Cell and Tissue Kinetics·J R BickenbachI C Mackenzie
May 1, 1984·American Journal of Ophthalmology·G O WaringM B Ekins
Aug 1, 1981·Journal of Dental Research·J R Bickenbach
Jul 15, 1981·Cancer·R SinghA Mittelman
Apr 1, 1994·The British Journal of Surgery·G T DeansR A Spence
Feb 26, 2000·Science·F M Watt, B L Hogan
Dec 21, 2000·The Journal of Cell Biology·J R BeauchampP S Zammit
Jun 21, 2001·American Journal of Respiratory Cell and Molecular Biology·D W BorthwickS H Randell
Jan 30, 2002·The British Journal of Ophthalmology·Penelope A McKelvieJohn D Santamaria
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·John Cairns
Jan 7, 2003·Genes & Development·Ariel A AvilionRobin Lovell-Badge
Mar 22, 2003·The Journal of Investigative Dermatology·Carol S TrempusRaymond W Tennant
Dec 13, 2003·Science·Tudorita TumbarElaine Fuchs
Mar 17, 2004·Nature Biotechnology·Rebecca J MorrisGeorge Cotsarelis
Mar 24, 2004·Cell·Elaine FuchsGeraldine Guasch
Sep 17, 2004·The Journal of Investigative Dermatology. Symposium Proceedings·Kristin M Braun, Fiona M Watt
Nov 9, 2004·Current Opinion in Cell Biology·Benjamin OhlsteinAllan Spradling
Dec 25, 2004·Developmental Biology·Robert B ClarkeChristopher S Potten
Dec 13, 2005·Journal of the American Society of Nephrology : JASN·Akito MaeshimaSanjay K Nigam
Dec 16, 2005·Nature·Zhicao YueCheng-Ming Chuong
Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Konrad UrbanekAnnarosa Leri
Jul 1, 2006·Science·Horace RheeElaine Fuchs
Nov 10, 2006·Nature·Jianlong WangStuart H Orkin
Mar 27, 2007·Differentiation; Research in Biological Diversity·Chunbo TengHongkui Deng
May 11, 2007·Annual Review of Cell and Developmental Biology·Maranke I Koster, Dennis R Roop
Oct 13, 2007·BMJ : British Medical Journal·Patrick Petignat, Michel Roy
Dec 1, 2007·Biochemical and Biophysical Research Communications·Yoshiaki KubotaToshio Suda
Mar 28, 2008·American Journal of Physiology. Renal Physiology·Eric A KurzrockRoslyn R Isseroff
May 6, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Reiichiro KuwaharaNeil D Theise
Jun 5, 2008·PloS One·Grant A Challen, Margaret A Goodell
Jul 3, 2008·Cell Stem Cell·Jonathan A NowakElaine Fuchs
Aug 20, 2008·Proceedings of the National Academy of Sciences of the United States of America·Paul P SzotekPatricia K Donahoe
Nov 7, 2008·Journal of Cell Science·Julie S Nielsen, Kelly M McNagny
Nov 13, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jin ZhaoTakayuki Nagasaki

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Citations

Jan 25, 2013·BMC Dermatology·Adrian J McNairnGéraldine Guasch
Sep 15, 2014·Pediatric Surgery International·Anita GuptaGéraldine Guasch
Jul 12, 2011·Stem Cell Reviews and Reports·Vasily V TerskikhEkaterina A Vorotelyak
Oct 7, 2014·Histopathology·Jean-François Fléjou
Apr 6, 2016·PloS One·Cassandra L MillerJames G Fox
Oct 24, 2017·Annual Review of Pathology·Dah-Jiun FuAlexander Yu Nikitin
Jul 1, 2014·Molecular & Cellular Oncology·Andrea Flesken-NikitinAlexander Yu Nikitin
Feb 15, 2014·Disease Models & Mechanisms·Laura A RunckGéraldine Guasch
Nov 8, 2014·Development·Heather A McCauleyGéraldine Guasch

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