Identification of erythroid-enriched gene expression in the mouse embryonic yolk sac using microdissected cells.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Latasha C RedmondJoyce A Lloyd

Abstract

Little is known about the genes that control the embryonic erythroid program. Laser capture microdissection was used to isolate primitive erythroid precursors and epithelial cells from frozen sections of the embryonic day 9.5 yolk sac. The RNA samples were amplified and labeled for hybridization to Affymetrix GeneChip Mouse Genome 430A 2.0 arrays. Ninety-one genes are expressed significantly higher in erythroid than in epithelial cells. Ingenuity pathway analysis indicates that many of these erythroid-enriched genes cluster in highly significant biological networks. One of these networks contains RBTN2/LMO2, SCL/TAL1, and EKLF/KLF1, three of the very few genes required for primitive erythropoiesis. Quantitative real-time polymerase chain reaction was used to verify that platelet factor 4, reelin, thrombospondin-1, and muscleblind-like 1 mRNA is erythroid-enriched. These genes have established roles in development or differentiation in other systems, and are, therefore, good candidates for regulating primitive erythropoiesis. These results provide a catalog of genes expressed during primitive erythropoiesis.

References

Apr 1, 1994·Molecular Biology of the Cell·J C Adams, J Lawler
Mar 1, 1995·Blood Reviews·M H EllisJ E Groopman
May 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M L Iruela-ArispeP Bornstein
Oct 23, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y Ikeda, T Terashima
Jan 1, 1997·Annual Review of Genetics·S H Orkin, L I Zon
Apr 16, 1998·The Journal of Clinical Investigation·J LawlerR O Hynes
Apr 23, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·R P TuckerC A Erickson
Sep 9, 2000·Current Opinion in Cell Biology·J Lawler
Jul 10, 2001·The Journal of Biological Chemistry·J J Bieker
Aug 10, 2001·Experimental Hematology·J Palis, M C Yoder
Nov 1, 2001·Annual Review of Cell and Developmental Biology·J C Adams
Dec 20, 2002·Bioinformatics·Earl HubbellRui Mei
Dec 10, 2003·Experimental Hematology·Margaret H Baron
Dec 13, 2003·Science·Rahul N KanadiaMaurice S Swanson
Jan 20, 2004·Development·Richard MartinTrang Hoang
Feb 13, 2004·Bioinformatics·Laurent GautierRafael A Irizarry
Sep 15, 2004·Clinical Chemistry·Catherine I DumurAndrea Ferreira-Gonzalez
Mar 22, 2005·The International Journal of Biochemistry & Cell Biology·Arne Slungaard
Apr 19, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Natalya V NarizhnevaTatiana V Byzova
Jun 1, 2005·Molecular and Cellular Biology·Roy DrissenSjaak Philipsen
Jul 12, 2005·Nature·Silvia GrisendiPier Paolo Pandolfi
Jul 28, 2005·Cytokine & Growth Factor Reviews·Robert M StrieterMichael P Keane
Aug 17, 2005·Child: Care, Health and Development·B Vachha, R Adams
Sep 6, 2005·Experimental Hematology·Kathleen E McGrath, James Palis
Oct 20, 2005·Blood·Nobuyuki MatsumotoScott L Friedman
Dec 29, 2005·Blood·Denise HodgeAndrew Perkins
Mar 15, 2006·Differentiation; Research in Biological Diversity·Maya PascualRuben Artero
May 24, 2006·Human Molecular Genetics·Xiaoyan LinCharles A Thornton
Dec 7, 2006·Developmental Neuroscience·Shanting ZhaoMichael Frotscher

❮ Previous
Next ❯

Citations

May 9, 2012·Molecular and Cellular Biology·Christopher J PangMarie Trudel
Mar 5, 2013·PloS One·Aditi R ChiplunkarJoyce A Lloyd
May 3, 2011·Blood Cells, Molecules & Diseases·Latasha C RedmondJoyce A Lloyd
Oct 17, 2015·BMC Developmental Biology·Ryan J CoramAndrea N Ladd
Dec 19, 2012·Cellular Immunology·Mirna Azalea-RomeroJulio Roberto Cáceres-Cortés
Oct 16, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Elvira Khialeeva, Ellen M Carpenter
Dec 8, 2011·Meditsinskaia tekhnika·D A Muslimov, A S Leliukhin
May 12, 2019·International Journal of Molecular Sciences·Víctor M Salinas-TorresLaura E Martínez de Villarreal
Mar 18, 2015·Statistical Applications in Genetics and Molecular Biology·Patrick McNuttPhil Beske
May 26, 2017·Nucleic Acids Research·Melissa D IlsleyAndrew C Perkins

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.