Identification of essential residues in the type II Hsp40 Sis1 that function in polypeptide binding.

The Journal of Biological Chemistry
Soojin LeeD Cyr

Abstract

Sis1 is an essential yeast Type II Hsp40 protein that assists cytosolic Hsp70 Ssa1 in the facilitation of processes that include translation initiation, the prevention of protein aggregation, and proteasomal protein degradation. An essential function of Sis1 and other Hsp40 proteins is the binding and delivery of non-native polypeptides to Hsp70. How Hsp40s function as molecular chaperones is unknown. The crystal structure of a Sis1 fragment that retains peptide-binding activity suggests that Type II Hsp40s utilize hydrophobic residues located in a solvent-exposed patch on carboxyl-terminal domain I to bind non-native polypeptides. To test this model, amino acid residues Val-184, Leu-186, Lys-199, Phe-201, Ile-203, and Phe-251, which form a depression in carboxyl-terminal domain I, were mutated, and the ability of Sis1 mutants to support cell viability and function as molecular chaperones was examined. We report that Lys-199, Phe-201, and Phe-251 are essential for cell viability and required for Sis1 polypeptide binding activity. Sis1 I203T could support normal cell growth, but when purified it exhibited severe defects in chaperone function. These data identify essential residues in Sis1 that function in polypeptide binding and...Continue Reading

References

Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K LiberekM Zylicz
Aug 1, 1991·The Journal of Cell Biology·A J Caplan, M G Douglas
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S WicknerK McKenney
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Jul 1, 1987·Molecular and Cellular Biology·M Werner-WashburneE A Craig
Jan 1, 1987·Methods in Enzymology·J D BoekeG R Fink
Jan 1, 1980·Molecular & General Genetics : MGG·C P GeorgopoulosM Feiss
Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·S J Landry, L M Gierasch
Apr 1, 1994·Trends in Biochemical Sciences·D M CyrM G Douglas
Feb 15, 1994·Biochemistry·T E Creighton, J J Ewbank
Jan 19, 1996·The Journal of Biological Chemistry·T ZhongK T Arndt
Jun 21, 1996·The Journal of Biological Chemistry·B BaneckiM Zylicz
Aug 9, 1996·The Journal of Biological Chemistry·Y MinamiF U Hartl
Jul 12, 1996·Journal of Molecular Biology·Y Q QianD J McColl
Feb 26, 1998·Cell·B Bukau, A L Horwich
May 19, 1998·Cell Stress & Chaperones·M E Cheetham, A J Caplan
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M K GreeneS J Landry
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·W C SuhC A Gross
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·C S GässlerB Bukau
Oct 19, 1999·Molecular and Cellular Biology·W Yan, E A Craig
Jul 6, 2000·Nature Structural Biology·M P MayerB Bukau
Nov 21, 2000·Current Biology : CB·W E GallT R Graham
Mar 27, 2001·The Journal of Cell Biology·J L Johnson, E A Craig
May 15, 2001·The EMBO Journal·N SondheimerS Lindquist

❮ Previous
Next ❯

Citations

Oct 6, 2006·Nature Structural & Molecular Biology·Peter C StirlingMichel R Leroux
Jun 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Pradeep KotaNikolay V Dokholyan
Aug 24, 2010·The Journal of Biological Chemistry·Judit Perales-CalvoFernando Moro
Dec 6, 2008·The Journal of Biological Chemistry·Daniel W SummersDouglas M Cyr
Dec 6, 2003·Molecular Biology of the Cell·Chun-Yang FanDouglas M Cyr
Oct 10, 2012·Molecular and Cellular Biology·Denis A KiktevYury O Chernoff
Jul 2, 2003·Molecular and Cellular Biology·Harm H KampingaCam Patterson
Apr 30, 2004·Cell Stress & Chaperones·Chun-Yang FanDouglas M Cyr
Aug 28, 2012·Current Pharmaceutical Design·Victoria A AssimonJason E Gestwicki
Sep 24, 2014·Biochimica Et Biophysica Acta·Nunziata Maio, Tracey A Rouault
Oct 27, 2015·Frontiers in Molecular Biosciences·Nadinath B Nillegoda, Bernd Bukau
May 31, 2003·EMBO Reports·Peter C StirlingMichel R Leroux
Mar 13, 2014·Cell Metabolism·Nunziata MaioTracey A Rouault
Jul 7, 2011·Virus Research·Caroline KnoxEva-Rachele Pesce
Jan 28, 2006·FEMS Microbiology Reviews·James P BurnieRuth C Matthews
Oct 21, 2014·PLoS Genetics·Michael ReidyDaniel C Masison
Nov 21, 2015·BioMed Research International·Lina ZhangRuntao Yang
Aug 23, 2003·The Journal of Biological Chemistry·Zhen ZhaoChih-Chen Wang
Apr 11, 2018·Journal of Biochemistry·Tomoya Uchida, Masaaki Kanemori
Oct 22, 2004·The Journal of Biological Chemistry·Chun-Yang FanDouglas M Cyr
Mar 15, 2006·The Journal of Biological Chemistry·Gordana Cogelja CajoPierre Genevaux
Jun 5, 2007·The Journal of Biological Chemistry·Meredith F N RosserDouglas M Cyr
Sep 16, 2006·The Journal of Biological Chemistry·Jeremy G BirdSue Wickner
Jun 2, 2006·The Biochemical Journal·Jingzhi LiBingdong Sha
Oct 8, 2016·Metallomics : Integrated Biometal Science·N Maio, T A Rouault
Apr 17, 2019·Life Science Alliance·David F MorenoMartí Aldea
Jan 11, 2020·Frontiers in Microbiology·Shih-Han KoWoan-Yuh Tarn
Jun 30, 2019·Nature Reviews. Molecular Cell Biology·Rina RosenzweigBernd Bukau
Jul 21, 2006·The Journal of Biological Chemistry·Nela S Cintron, David Toft
Jan 19, 2021·Experimental Cell Research·Sara María Ayala Mariscal, Janine Kirstein

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