Identification of expanded T-cell clones by spectratyping in nonfunctioning kidney transplants

Journal of Inflammation Research
Maria CappuccilliGaetano La Manna

Abstract

The aim of this study was the application of complementarity-determining region-3 spectratyping analysis to determine T-cell-repertoire complexity and to detect T-cell-clone expansion, as a measure of immune response in nonfunctioning kidney transplants (group hemodialysis-transplant [HD-Tx]), nontransplanted dialysis patients (group hemodialysis [HD]), and normal subjects as controls (group C). Analysis of T-cell receptor (TCR) diversity by spectratyping was applied to peripheral blood samples collected from 21 subjects: eight in group HD-Tx, seven in group HD, and six in group C. Considering the extent of the skew in TCR variable region repertoires as a measure of clonal T cells, we found that the number of altered spectra showed a progressive increase from normal subjects to dialysis patients and to nonfunctioning kidney transplants, respectively. Healthy subjects had the lowest number of altered spectra, and patients with nonfunctioning kidney transplants the highest. Differences were significant for group HD-Tx vs group C (P=0.017) and group HD vs group C (P=0.015), but not between nonfunctioning kidney-transplant recipients and dialysis patients (group HD-Tx vs group HD). Although dialysis appears to be a weaker trigger f...Continue Reading

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PCR
electrophoresis
biopsy

Software Mentioned

Statistical Package for the Social Sciences ( SPSS )
GeneScan
Genotyper

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