PMID: 6170319Aug 18, 1981Paper

Identification of exposed and buried determinants of the membrane-bound acetylcholine receptor from Torpedo californica

Biochemistry
S C Froehner

Abstract

The ability of five rabbit anti-acetylcholine receptor antisera to recognize the membrane-bound receptor from Torpedo californica has been investigated. Two antisera, raised against affinity-purified native receptor, react extensively with purified receptor-rich membrane vesicles. Since the membrane vesicles are impermeable to macromolecules and are oriented right side out, these two antisera recognize predominantly extracellular determinants. Two antisera against sodium dodecyl sulfate denatured receptor and one against purified delta subunit react poorly with the membrane-bound receptor. Only 10-20% of the determinants recognized by these antisera are accessible to antibodies when the receptor is membrane bound. Many of the latent sites can be exposed by permeabilizing the vesicles with saponin, by alkaline extraction of the membranes to remove peripheral proteins, or by a combination of these two treatments. These treatments neither solubilize the receptors nor interfere with their ability to undergo agonist-induced affinity changes. Subunit analysis of the sites on the membrane-bound receptor that are accessible to antibodies indicates that the alpha, beta, and delta chains possess extracellular determinants. Buried sites a...Continue Reading

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Citations

Mar 15, 2000·Muscle & Nerve·J M Lindstrom
Nov 1, 1988·Journal of the Neurological Sciences·S KawanamiM A Raftery
Jan 1, 1983·Progress in Neurobiology·J J McArdle
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Jun 25, 1984·Journal of Molecular Biology·F BonJ P Changeux
Sep 21, 1984·Science·J P ChangeuxP Chemouilli

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