Identification of Factors Complicating Bioluminescence Imaging

Biochemistry
Hsien-Wei YehHui-Wang Ai

Abstract

In vivo bioluminescence imaging (BLI) has become a standard, non-invasive imaging modality for following gene expression or the fate of proteins and cells in living animals. Currently, bioluminescent reporters used in laboratories are mostly derivatives of two major luciferase families: ATP-dependent insect luciferases and ATP-independent marine luciferases. Inconsistent results of experiments using different bioluminescent reporters, such as Akaluc and Antareas2, have been reported. Herein, we re-examined the inconsistency in several experimental settings and identified the factors, such as ATP dependency, stability in serum, and molecular sizes of luciferases, that contributed to the observed differences. We expect this study will make the research community aware of these factors and facilitate more accurate interpretation of BLI data by considering the nature of each bioluminescent reporter.

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Citations

Nov 26, 2019·Chemistry : a European Journal·Eloi P CoutantYves L Janin
Nov 27, 2020·Biochemical Society Transactions·Lieuwe BiewengaMaarten Merkx
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Oct 9, 2019·Accounts of Chemical Research·Sierra J Williams, Jennifer A Prescher
Apr 11, 2019·ACS Chemical Biology·Hsien-Wei YehHui-Wang Ai

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