Identification of flagellar proteins that initiate the activation of sperm motility in vivo

Biochemical and Biophysical Research Communications
G E BrachoJ S Tash

Abstract

Protein phosphorylation appears to be a necessary step in the intracellular signaling pathway that initiates the activation of sperm motility. Activation of live immotile sea urchin sperm produced rapid, time-dependent increased phosphorylation on proteins of 32, 45, 130, and 500 kDa. Fractionation of immotile and motile sperm indicated that these motility-related phosphoproteins are associated with flagella. These proteins showed greater phosphorylation in the flagellar fraction from motile sperm, suggesting that subcellular boundaries are in place to keep protein kinases and their substrates spatially separated. Solubility properties suggest that these proteins are the heavy chain and smaller subunits of sea urchin sperm dynein which are phosphorylated in vivo to initiate activation of motility. This also suggests that phosphorylation of only these few proteins, out of the nearly 100 phosphorylations known to occur in the basic axoneme, appears to be associated with the early signaling pathways of motility activation in intact sperm.

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Citations

Nov 18, 2003·Reproductive Biology and Endocrinology : RB&E·Ralf R Henkel, Wolf-Bernhard Schill
Oct 3, 2002·Biochemical and Biophysical Research Communications·Kazuo Inaba
Oct 30, 1998·Biochemical and Biophysical Research Communications·J S Tash, G E Bracho
Aug 26, 2014·Reproductive Biology·Viktoriya Dzyuba, Jacky Cosson
Aug 10, 2004·Reproductive Medicine and Biology·Masakatsu FujinokiMakoto Okuno
Jun 3, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J S Tash, G E Bracho
Jul 3, 2019·Theriogenology·Sayyed Mohammad Hadi AlaviOtomar Linhart
Nov 6, 2007·The Journal of Experimental Biology·Daniel WhiteClaude Gagnon
Feb 28, 2014·The Journal of Experimental Biology·Martin TresguerresJinae N Roa
Mar 6, 1999·Biochemical and Biophysical Research Communications·K InabaK Ogawa
Jan 28, 2003·The Journal of Experimental Biology·M MoritaM Okuno
May 28, 2003·Biochemical and Biophysical Research Communications·Atsuko ItohMasaaki Morisawa
Jun 28, 2005·Gene·Mamoru NomuraVictor D Vacquier
Dec 3, 2021·Zygote : the Biology of Gametes and Early Embryos·Arlet Loza-HuertaCarmen Beltrán

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