Identification of four evolutionarily related G protein-coupled receptors from the malaria mosquito Anopheles gambiae

Biochemical and Biophysical Research Communications
Martin BelmontCornelis J P Grimmelikhuijzen

Abstract

The mosquito Anopheles gambiae is an important vector for malaria, which is one of the most serious human parasitic diseases in the world, causing up to 2.7 million deaths yearly. To contribute to our understanding of A. gambiae and to the transmission of malaria, we have now cloned four evolutionarily related G protein-coupled receptors (GPCRs) from this mosquito and expressed them in Chinese hamster ovary cells. After screening of a library of thirty-three insect or other invertebrate neuropeptides and eight biogenic amines, we could identify (de-orphanize) three of these GPCRs as: an adipokinetic hormone (AKH) receptor (EC(50) for A. gambiae AKH, 3x10(-9)M), a corazonin receptor (EC(50) for A. gambiae corazonin, 4x10(-9)M), and a crustacean cardioactive peptide (CCAP) receptor (EC(50) for A. gambiae CCAP, 1x10(-9)M). The fourth GPCR remained an orphan, although its close evolutionary relationship to the A. gambiae and other insect AKH receptors suggested that it is a receptor for an AKH-like peptide. This is the first published report on evolutionarily related AKH, corazonin, and CCAP receptors in mosquitoes.

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Citations

Mar 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Elisabeth StafflingerCornelis J P Grimmelikhuijzen
Jan 14, 2010·The Journal of Biological Chemistry·Karina K HansenCornelis J P Grimmelikhuijzen
Oct 1, 2009·Integrative and Comparative Biology·Matthias W Lorenz, Gerd Gäde
Dec 24, 2013·Molecular Biology and Evolution·Graeme J RochNancy M Sherwood
Aug 12, 2014·General and Comparative Endocrinology·Graeme J RochNancy M Sherwood
May 3, 2011·Journal of Insect Science·R ZieglerM A Wells
May 27, 2014·General and Comparative Endocrinology·Ravisankar Valsalan, Narayanan Manoj
Jul 25, 2014·General and Comparative Endocrinology·Frank Hauser, Cornelis J P Grimmelikhuijzen
Apr 24, 2015·Open Biology·Dean C SemmensMaurice R Elphick
Jul 15, 2015·Insect Biochemistry and Molecular Biology·Michele Alves-BezerraKatia C Gondim
Sep 16, 2016·Scientific Reports·Shizhong LiCornelis J P Grimmelikhuijzen
Jun 18, 2017·General and Comparative Endocrinology·Meet ZandawalaMaurice R Elphick
Feb 15, 2018·International Journal of Molecular Sciences·Elisabeth MarchalHeleen Verlinden
Feb 1, 2013·The Journal of Experimental Biology·Tania Y Estévez-LaoJulián F Hillyer
Jun 8, 2021·RSC Medicinal Chemistry·Ashlin TurnerDavid J Craik
Dec 7, 2007·Frontiers in Neuroendocrinology·Frank HauserCornelis J P Grimmelikhuijzen
Oct 10, 2009·Insect Biochemistry and Molecular Biology·Jan A Veenstra
Dec 28, 2010·General and Comparative Endocrinology·Graeme J RochNancy M Sherwood

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