Identification of Four-Jointed Box 1 (FJX1)-Specific Peptides for Immunotherapy of Nasopharyngeal Carcinoma

PloS One
San Jiun ChaiKue Peng Lim

Abstract

Nasopharyngeal carcinoma (NPC) is highly prevalent in South East Asia and China. The poor outcome is due to late presentation, recurrence, distant metastasis and limited therapeutic options. For improved treatment outcome, immunotherapeutic approaches focusing on dendritic and autologous cytotoxic T-cell based therapies have been developed, but cost and infrastructure remain barriers for implementing these in low-resource settings. As our prior observations had found that four-jointed box 1 (FJX1), a tumor antigen, is overexpressed in NPCs, we investigated if short 9-20 amino acid sequence specific peptides matching to FJX1 requiring only intramuscular immunization to train host immune systems would be a better treatment option for this disease. Thus, we designed 8 FJX1-specific peptides and implemented an assay system to first, assess the binding of these peptides to HLA-A2 molecules on T2 cells. After, ELISPOT assays were used to determine the peptides immunogenicity and ability to induce potential cytotoxicity activity towards cancer cells. Also, T-cell proliferation assay was used to evaluate the potential of MHC class II peptides to stimulate the expansion of isolated T-cells. Our results demonstrate that these peptides ar...Continue Reading

References

Nov 15, 1977·International Journal of Cancer. Journal International Du Cancer·P A TrumperS Finerty
Aug 22, 1973·Nature: New Biology·H WolfV Becker
Feb 1, 1967·The Journal of Laryngology and Otology·C S Muir, W F Oakley
Mar 1, 1996·International Immunology·F A VerreckF Koning
Sep 1, 1996·Transfusion·D C ShiehK J Kao
Jul 1, 1996·Journal of Immunotherapy with Emphasis on Tumor Immunology : Official Journal of the Society for Biological Therapy·F M MarincolaS A Rosenberg
Jan 1, 1997·Annual Review of Immunology·A B Rickinson, D J Moss
Apr 29, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M Al-SarrafJ F Ensley
Oct 31, 1998·Current Opinion in Immunology·D M Pardoll, S L Topalian
Jun 8, 1999·Developmental Biology·C Rauskolb, K D Irvine
Aug 17, 1999·International Journal of Cancer. Journal International Du Cancer·S T CheungJ C Lee
Dec 22, 1999·Immunogenetics·H RammenseeS Stevanović
Jun 6, 2002·Journal of the National Cancer Institute·Giorgio ParmianiAndrea Anichini
Jun 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Neil A MarshallRobert N Barker
Jan 9, 2004·International Journal of Cancer. Journal International Du Cancer·Olivier FaureKostas Kosmatopoulos
Aug 3, 2004·Nature Reviews. Immunology·Michael J Bevan
Sep 24, 2004·Journal of Translational Medicine·Kimberly A Shafer-WeaverAnatoli Malyguine
Mar 23, 2005·Immunity·Alexandre CorthayBjarne Bogen
Sep 8, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Rebecca RockManfred Gessler
Sep 15, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·George E PeoplesSathibalan Ponniah
Mar 1, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ronald J BuckanovichGeorge Coukos
Jun 5, 2007·Cancer Treatment Reviews·Amine MasmoudiMounir Frikha
Oct 18, 2007·International Journal of Radiation Oncology, Biology, Physics·Brian O'Sullivan
Jan 16, 2008·International Journal of Cancer. Journal International Du Cancer·Sylvia Yat-Yee ChanKwok-Wai Lo
Feb 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·George E PeoplesSathibalan Ponniah
Jul 19, 2008·Science·Hiroyuki O IshikawaKenneth D Irvine
Jan 15, 2009·The Journal of Pathology·Shikha BosePaul G Murray
Aug 8, 2009·EMBO Reports·Francisco Jose Gutierrez-AviñoMaria Dominguez
Dec 30, 2009·Chinese Journal of Cancer·Jia-Xin LiWei-Wei Xiao
Mar 4, 2010·Tissue Antigens·J S DhaliwalY Y Lee
May 11, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Albiruni R A RazakKelvin Chan
May 21, 2010·Cancer Investigation·Nasrollah ErfaniAbbas Ghaderi

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Citations

Sep 8, 2018·Human Vaccines & Immunotherapeutics·San Jiun ChaiKue Peng Lim
May 2, 2020·Cancer Biotherapy & Radiopharmaceuticals·Neng WanJing Wang
Jun 27, 2019·Disease Markers·San Jiun ChaiSok Ching Cheong
Jul 20, 2019·Medical Oncology·Ali Mohamed AlshabiChanabasayya Vastrad
Mar 25, 2021·The Journal of Biological Chemistry·Carolyn A WorbySourav Banerjee

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Methods Mentioned

BETA
PCR
biopsy
flow cytometry
ELISA
biopsies

Software Mentioned

SPSS
ImmunoSpot Professional
SYFPEITHI
SYFPEITHI Epitope Prediction
BIMAS
BIMAS Peptide Binding Predictions

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