PMID: 2147946Dec 1, 1990Paper

Identification of four subsets of human CD3-CD16+ natural killer (NK) cells by the expression of clonally distributed functional surface molecules: correlation between subset assignment of NK clones and ability to mediate specific alloantigen recognition

The Journal of Experimental Medicine
A MorettaE Ciccone

Abstract

In previous studies we identified a surface molecule (termed GL183) capable of mediating cell activation and selectively expressed by a subset of human CD3-CD16+ natural killer (NK) cells. In this study we analyzed whether other subset-specific functional molecules were expressed in GL183- NK cells. To this end, mice were immunized with the PE29 (CD3-CD16+GL183-) NK clone. Monoclonal antibodies (mAbs) were selected by screening the hybridoma supernatants for their ability to trigger the cytolytic activity of clone PE29 against the human myelomonocytic leukemia U937. The EB6 mAb (IgG1) triggered the PE29 clone, but not a GL183+ clone used as a control. EB6+ cells ranged between 1 and 13% of peripheral blood lymphocytes and were largely included in the CD3-CD16+CD56+ cell populations (only less than 2% of EB6+ cells were CD3+). Analysis of resting or activated CD3-CD16+ populations, or clones for the expression of EB6 or GL183 mAbs, allowed us to identify four distinct, phenotypically stable, NK subsets (EB6+GL183-; EB6+GL183+; EB6-GL183+; EB6-GL183-). Similar to GL183 mAb, the EB6 mAb selectively triggered the NK subset expressing the corresponding surface antigen to lyse human tumor cell lines including U937, IGROV-I, M14, and ...Continue Reading

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Citations

Sep 1, 1996·Journal of Clinical Immunology·L MorettaA Moretta
Jan 1, 1994·International Journal of Clinical & Laboratory Research·L MorettaA Moretta
Aug 5, 2003·International Journal of Hematology·Sherif S FaragMichael A Caligiuri
Jan 5, 2006·Immunogenetics·Anita E TrundleyAshley Moffett
Oct 20, 1994·Mechanisms of Ageing and Development·E MarianiA Facchini
Jul 1, 1992·Immunology Today·R Versteeg
Aug 1, 1992·Immunology Today·L MorettaK Kärre
Feb 1, 1996·Immunology Today·L L Lanier, J H Phillips
Sep 1, 1993·Human Immunology·M ThomsenF T Christiansen
Feb 1, 1992·Current Opinion in Immunology·L L Lanier, J H Phillips
Feb 1, 1993·Current Opinion in Immunology·W M Yokoyama
Feb 1, 1995·Current Opinion in Immunology·W M Yokoyama
Mar 16, 2005·Current Opinion in Immunology·Loredana RuggeriAndrea Velardi
Dec 3, 2013·Computers in Biology and Medicine·Juan C Cuevas TelloChristian A García-Sepúlveda
Aug 28, 2004·Comptes rendus biologies·Roberta CastriconiAlessandro Moretta
Nov 18, 1998·Journal of Immunological Methods·C FrohnH Kirchner
Apr 17, 2002·Molecular Immunology·Jeffrey C Boyington, Peter D Sun
Aug 1, 1997·Molecular Immunology·L Jaso-FriedmannD L Evans
Sep 9, 1999·Journal of Reproductive Immunology·C CantoniR Biassoni
Feb 5, 2000·Human Immunology·M Valés-GómezJ Strominger
Feb 5, 2000·Human Immunology·A De Maria, L Moretta
Feb 1, 1996·Current Opinion in Immunology·M Colonna
Jun 1, 1996·Current Opinion in Immunology·D H Raulet
Nov 22, 1997·Current Opinion in Immunology·A Moretta, L Moretta
Feb 1, 1997·Current Opinion in Immunology·L L Lanier
Sep 10, 2002·Transplant Immunology·Derek MiddletonLynne Maxwell
Dec 31, 2002·Microbes and Infection·Lorenzo MorettaAlessandro Moretta
Aug 30, 2002·Trends in Immunology·Andrea VelardiLorenzo Moretta
Mar 25, 2004·Nature Reviews. Immunology·Eric Vivier, Nicolas Anfossi
Apr 18, 2007·Nature Reviews. Immunology·Hans-Gustaf Ljunggren, Karl-Johan Malmberg
Feb 23, 2007·Genes and Immunity·G E DaviesS K Anderson

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