Identification of four trp1 gene variants murine pancreatic beta-cells

Diabetologia
H Sakura, F M Ashcroft

Abstract

Insulin secretion is stimulated by glucose, hormones and neurotransmitters. Both activation of a non-selective cation current and activation of a Ca2+ current in response to depletion of intracellular Ca2+ stores have been suggested to play a role in this stimulation. The properties of these currents resemble those reported for the Drosophila genes trp and trpl. Using the reverse transcription polymerase chain reaction and Northern blot analysis we found that of the six mammalian trp-related genes (trp1-6), only trp1 was expressed at high levels in the mouse insulinoma cell line MIN6. We cloned the murine homologue of human trp1 from MIN6 cells and identified four variants (alpha, beta, gamma and delta), generated by alternative splicing near the N-terminus of the protein. In vitro translation showed that only the alpha and beta splice variants are efficiently expressed. The beta variant is the dominant form in MIN6 cells (and probably in mouse pancreatic islets), whereas the alpha variant is the major type in the mouse brain. The beta variant showed 99% identity to the human homologue at the amino acid level.

Citations

Mar 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J W Putney, R R McKay
Apr 28, 2005·Naunyn-Schmiedeberg's Archives of Pharmacology·Annette LisStephan E Philipp
Nov 3, 2004·The Journal of Membrane Biology·David Mears
Oct 14, 2004·Pflügers Archiv : European journal of physiology·Joris VriensBernd Nilius
May 27, 2003·Cell Calcium·D J BeechR Flemming
Apr 19, 2002·Progress in Neurobiology·Christof ZittAndreas Lückhoff
Jun 9, 1999·The Biochemical Journal·L K BobanovicM D Bootman
Jul 15, 2003·Respirology : Official Journal of the Asian Pacific Society of Respirology·Hwei L OngGreg J Barritt
Aug 4, 1998·The EMBO Journal·S PhilippV Flockerzi
Feb 25, 2005·Science's STKE : Signal Transduction Knowledge Environment·Craig Montell
Feb 8, 2006·Annual Review of Physiology·Grzegorz OwsianikBernd Nilius
Mar 3, 2012·Biological & Pharmaceutical Bulletin·Satoshi NumazawaTakemi Yoshida
Sep 23, 2014·Cell Calcium·Patrick GilonMagalie A Ravier
Feb 21, 2016·Pharmacology & Therapeutics·Susumu OhyaKatsuhiko Muraki
May 27, 2003·The Journal of Physiology·L H ClarsonT Powell
Dec 19, 2006·Seminars in Cell & Developmental Biology·Esther Vázquez, Miguel A Valverde
Jan 5, 2011·British Journal of Pharmacology·Chihab KloseChristian Harteneck
Jan 26, 2012·Islets·Amanda Jabin FågelskiöldMd Shahidul Islam
Dec 26, 2001·Science's STKE : Signal Transduction Knowledge Environment·C Montell
Oct 1, 2014·Cells·Alexander DietrichThomas Gudermann
Jan 5, 2011·Diabetes·Patrick E MacDonald
Dec 2, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Amanda Jabin GustafssonMd Shahidul Islam
Aug 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J L BurnsA B Hassan
Feb 1, 2000·The Journal of Biological Chemistry·X LiuI S Ambudkar
Nov 14, 1997·Molecular Pharmacology·C Montell
Mar 11, 2000·American Journal of Physiology. Cell Physiology·X WuM L Villereal
Jun 19, 2013·The Journal of Biological Chemistry·E-Ching OngLeonidas Tsiokas

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