Identification of FOXO targets that generate diverse features of the diapause phenotype in the mosquito Culex pipiens

Proceedings of the National Academy of Sciences of the United States of America
Cheolho SimDavid L Denlinger

Abstract

Insulin and juvenile hormone signaling direct entry of the mosquito Culex pipiens into its overwintering adult diapause, and these two critical signaling pathways appear to do so by converging on the regulation of forkhead transcription factor (FOXO). Diapause is a complex phenotype, and FOXO emerges as a prime candidate for activating many of the diverse physiological pathways that generate the diapause phenotype. Here, we used ChIP sequencing to identify direct targets of FOXO. The nearest gene in a 10-kb region surrounding a predicted binding site was extracted for each binding site, resulting in a dataset containing genes potentially regulated by FOXO. By selecting candidate genes based on their functional relevance to diapause, we identified five gene categories of potential interest, including stress tolerance, metabolic pathways, lifespan extension, cell cycle and growth regulation, and circadian rhythms. Twelve targets were prioritized for further analysis, 10 of which were validated by ChIP-quantitative PCR and quantitative real-time PCR. These 10 genes activated by FOXO are highly up-regulated during diapause and are thus strong candidates for implementation of the diapause syndrome.

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Citations

Jan 18, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Shanshan XingHong Yin
Dec 24, 2015·Insect Biochemistry and Molecular Biology·Xinda LinLaura Corley Lavine
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Jul 22, 2017·Molecular & Cellular Proteomics : MCP·Tamar ZivEsther Lubzens
Mar 9, 2018·The Journal of Experimental Biology·Brent J Sinclair, Katie E Marshall
Aug 30, 2017·Proceedings of the National Academy of Sciences of the United States of America·Xiao-Shuai ZhangWei-Hua Xu
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Apr 8, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Steven C HandRichard Roy
Dec 6, 2018·Frontiers in Physiology·Zhen-Juan YinYi-Fan Zhai
Apr 16, 2019·Frontiers in Endocrinology·Arvind SharmaMonika Gulia-Nuss
Jul 20, 2017·Proceedings of the National Academy of Sciences of the United States of America·Vladimír KoštálAlexander William Bruce
Oct 20, 2020·Frontiers in Cell and Developmental Biology·Siyuan GuoLe Kang
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Apr 17, 2021·Archives of Insect Biochemistry and Physiology·Bo ZhangAi-Ping Liu
Apr 17, 2021·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Bryan KingCheolho Sim
Apr 28, 2021·Genome Biology and Evolution·Xingzhou MaXue-Xin Chen

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