Identification of free radicals by spin trapping with DEPMPO and MCPIO using tandem mass spectrometry

European Journal of Mass Spectrometry
Ana ReisP Domingues

Abstract

This study evaluates the use of a pyrroline (DEPMPO) and an imidazole (MCPIO) spin trap for the detection of hydroxyl and biomolecule (a peptide and a phospholipid) free radical adducts by Electrospray Ionization Mass Spectrometry (ESI-MS). The hydroxyl and biomolecule free radical adducts were detected using a QTOF and a linear ion trap (LIT) mass spectrometers. In the presence of hydroxyl radical, the mass spectrum obtained for each of the spin traps, DEPMPO and MCPIO, showed the presence of ions that could be attributed to hydroxyl and peroxyl radicals. Further characterisation by tandem mass spectrometry (ESI-MS/MS) revealed also the presence of hydroxy-hydroxyl adducts. Based on the results here described, we show that DEPMPO is a better spin trap for free radicals trapping and detection by mass spectrometry mainly because adducts show increased signal intensity. The ESI-MS spectra obtained for DEPMPO and MCPIO in the presence of biomolecule radicals (peptide and phospholipid) show molecular ions of DEPMPO and MCPIO adducts, which were characterised by tandem mass spectrometry. Both carbon centered radicals and oxygen centered radicals were efficiently trapped by the two spin traps and analysis of QTOF-MS/MS mass spectra a...Continue Reading

References

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Citations

Jun 4, 2013·Biochimica Et Biophysica Acta·Angela LoganMichael P Murphy
Apr 10, 2013·Biochimica Et Biophysica Acta·Clare L Hawkins, Michael J Davies
Mar 8, 2012·Rapid Communications in Mass Spectrometry : RCM·Cláudia SimõesMaria Rosario M Domingues

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