PMID: 20640387Jul 20, 2010Paper

Identification of fungemia agents using the polymerase chain reaction and restriction fragment length polymorphism analysis

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
M S SantosJ V B Souza

Abstract

Prompt and specific identification of fungemia agents is important in order to define clinical treatment. However, in most cases conventional culture identification can be considered to be time-consuming and not without errors. The aim of the present study was to identify the following fungemia agents: Candida albicans, Candida parapsilosis, Candida tropicalis, Candida glabrata, Cryptococcus neoformans, Cryptococcus gattii, and Histoplasma capsulatum using the polymerase chain reaction and restriction fragment length polymorphism analysis (PCR/RFLP). More specifically: a) to evaluate 3 different amplification regions, b) to investigate 3 different restriction enzymes, and c) to use the best PCR/RFLP procedure to indentify 60 fungemia agents from a culture collection. All 3 pairs of primers (ITS1/ITS4, NL4/ITS5 and Primer1/Primer2) were able to amplify DNA from the reference strains. However, the size of these PCR products did not permit the identification of all the species studied. Three restriction enzymes were used to digest the PCR products: HaeIII, Ddel and Bfal. Among the combinations of pairs of primers and restriction enzymes, only one (primer pair NL4/ITS5 and restriction enzyme Ddel) produced a specific RFLP pattern f...Continue Reading

References

Jan 1, 1991·Journal of Clinical Microbiology·K V ClemonsD A Stevens
Nov 1, 1993·Journal of Clinical Microbiology·E Bart-DelabesseB Dupont
Feb 13, 1993·BMJ : British Medical Journal·A F Markham
Jan 1, 1994·Journal of Clinical Microbiology·A R HolmesH F Jenkinson
Dec 1, 1993·Journal of Clinical Microbiology·Y MiyakawaY Fukazawa
Jun 1, 1997·Journal of Clinical Microbiology·H EinseleU Schumacher
Nov 30, 2000·Current Infectious Disease Reports·C M Toscano, W R Jarvis
Sep 28, 2001·Journal of Clinical Microbiology·H C ChangT C Chang
Aug 1, 2002·Journal of Clinical Microbiology·Guizhen Luo, Thomas G Mitchell
Apr 18, 2003·The New England Journal of Medicine·Greg S MartinMarc Moss
Jan 28, 2004·Journal of Microbiological Methods·Anja TrostThomas Adam
Aug 6, 2005·Journal of Clinical Microbiology·Paula SampaioCélia Pais

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