Abstract
Mammalian glycan chain elongation is mostly based on extending the type 2 chain, Galbeta1-4GlcNAc, whereas the corresponding type 1 chain, Galbeta1-3GlcNAc, is not normally extended. In a broader context of developing high sensitivity mass spectrometry methodologies for glycomic identification of Le(a) versus Le(x) and linear versus branched poly-N-acetyllactosamine (polyLacNAc), we have now shown that the dimeric type 1 glycan chain, as carried on the lactosylceramides of a human colonic adenocarcinoma cell line, Colo205, not only can be further extended linearly but can likewise be branched at C6 of 3-linked Gal in a manner similar to polyLacNAc. A combination of chemical and enzymatic derivatization coupled with advanced mass spectrometry analyses afforded unambiguous identification of a complex mixture of type 1 and 2 hybrids as well as those fucosylated variants founded exclusively on linear and branched trimeric type 1 chain. We further showed by in vitro enzymatic synthesis that extended type 1 and the hybrid chains can be branched by all three forms of the human I branching enzymes (IGnT) currently identified but with lower efficiency and stringency with respect to branching site preference. Importantly, it was found th...Continue Reading
References
Feb 1, 1992·European Journal of Biochemistry·M R StroudS Hakomori
May 1, 1989·Archives of Biochemistry and Biophysics·E H Holmes
Jan 1, 1994·Methods in Enzymology·A DellH R Morris
Mar 27, 1999·The Journal of Biological Chemistry·M UjitaM Fukuda
Apr 23, 1999·The Journal of Biological Chemistry·S IsshikiH Narimatsu
May 18, 1999·Biological & Pharmaceutical Bulletin·S MutoK Matsumoto
Dec 2, 1999·Biochimica Et Biophysica Acta·J W DennisC E Warren
Apr 5, 2000·The Journal of Biological Chemistry·M UjitaM Fukuda
Oct 24, 2000·The Journal of Biological Chemistry·N ShiraishiK Sasaki
Apr 3, 2001·The Journal of Biological Chemistry·A TogayachiH Narimatsu
Nov 9, 2002·Blood·Lung-Chih YuMarie Lin
Dec 7, 2002·Blood·Niro InabaHisashi Narimatsu
Jul 2, 2003·Proteomics·Cornelia KoyMichael O Glocker
Sep 30, 2003·Glycobiology·Jonas AngströmStephen Henry
Feb 18, 2004·Rapid Communications in Mass Spectrometry : RCM·Emanuela SpinaMatthias Glueckmann
Jan 7, 2005·The Journal of Biological Chemistry·Motoki TeradaNobuko Kawasaki
Aug 2, 2005·Analytical Chemistry·Akihiko KameyamaHisashi Narimatsu
Jun 28, 2006·Biochemistry·Sheng-Wei LinChun-Hung Lin
Aug 10, 2006·Glycoconjugate Journal·Shin-Yi YuKay-Hooi Khoo
Nov 23, 2006·Methods in Enzymology·Akira TogayachiHisashi Narimatsu
Citations
Jan 13, 2009·Glycobiology·Chi-Hung LinKay-Hooi Khoo
Jan 9, 2009·Glycobiology·Nobuko KawasakiToshisuke Kawasaki
May 15, 2010·Glycobiology·Hasse KarlssonSusann Teneberg
Jul 13, 2012·Glycobiology·Wenjie PengNahid Razi
Nov 5, 2015·Analytical Chemistry·Chao GaoWengang Chai
Jul 18, 2009·Proteomics·Shin-Yi YuAlbert M Wu
Jun 10, 2011·Proteomics·Shui-Hua WangKay-Hooi Khoo
Aug 19, 2011·Mass Spectrometry Reviews·David J Harvey
Oct 23, 2008·Molecular & Cellular Proteomics : MCP·Chu-Wei KuoKay-Hooi Khoo
Aug 11, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thomas FischöderLothar Elling
Oct 23, 2015·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Somdutta SahaThomas Kieber-Emmons
Aug 17, 2011·International Journal of Molecular Sciences·Chee Sian KuanLing Ling Few
Jan 26, 2021·Biochemical Society Transactions·Kay-Hooi Khoo
Dec 16, 2020·The Journal of Organic Chemistry·Nitish VermaChun-Hung Lin
Mar 2, 2010·ACS Chemical Biology·Ching-Wen HoChun-Hung Lin