Identification of G protein-coupled receptors required for vitellogenesis and egg development in an insect with panoistic ovary.

Insect Science
Hongyuan ZhengShutang Zhou

Abstract

G protein-coupled receptors (GPCRs), a superfamily of integral transmembrane proteins regulate a variety of physiological processes in insects. Juvenile hormone (JH) is known to stimulate Vitellogenin (Vg) synthesis in the fat body, secretion into the hemolymph and uptake by developing oocytes. However, the role of GPCRs in JH-dependent insect vitellogenesis and oocyte maturation remains elusive. In the present study, we performed transcriptomic analysis and RNA interference (RNAi) screening in vitellogenic females of the migratory locust Locusta migratoria. Of 22 GPCRs identified in ovarian transcriptome, LGR4, OR-A1, OR-A2, Mthl1, Mthl5 and Smo were most abundant in the ovary. By comparison, mAChR-C expressed at higher levels in the fat body, whereas Oct/TyrR, OARβ, AdoR and ADGRA3 were at higher expression levels in the brain. Our RNAi screening demonstrated that knockdown of six GPCRs resulted in defective phenotypes of Vg accumulation in developing oocytes, accompanied by blocked ovarian development and impaired oocyte maturation. While LGR4 and Oct/TyrR appeared to control Vg synthesis in the fat body, OR-A1, OR-A2, mAChR-C and CirlL regulated Vg transportation and uptake. The findings provide fundamental evidence for dec...Continue Reading

References

Jul 26, 2000·The Journal of Cell Biology·T Brody, A Cravchik
Aug 25, 2001·Annual Review of Neuroscience·T C Südhof
Oct 5, 2002·Science·Catherine A HillLaurence J Zwiebel
Nov 14, 2002·Behavioural Brain Research·Ricarda ScheinerJoachim Erber
Jan 1, 2004·Molecular and Cellular Biology·Magda A M Krajnc-FrankenJan A Gossen
Apr 19, 2005·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Barbara GrajewskiMelissa A McDiarmid
May 14, 2005·Journal of Insect Physiology·Maciej A PszczolkowskiSonny B Ramaswamy
Jun 30, 2005·Archives of Insect Biochemistry and Physiology·Goudarz MolaeiAngela B Lange
Oct 31, 2006·Progress in Neurobiology·Frank HauserCornelis J P Grimmelikhuijzen
Dec 7, 2007·Frontiers in Neuroendocrinology·Frank HauserCornelis J P Grimmelikhuijzen
Aug 6, 2010·Insect Biochemistry and Molecular Biology·Yi FanNaiming Zhou
Dec 31, 2010·Advances in Experimental Medicine and Biology·Matthias B Van HielJozef Vanden Broeck
Dec 12, 2012·Frontiers in Endocrinology·Jelle CaersLiliane Schoofs
Mar 5, 2013·General and Comparative Endocrinology·Pieter Van WielendaeleJozef Vanden Broeck
Sep 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ting ZhangPing Shen
Oct 29, 2013·Annual Review of Entomology·Xianhui Wang, Le Kang
Feb 11, 2014·Cell Communication and Signaling : CCS·Mei-Juan CaiXiao-Fan Zhao
Jul 16, 2014·Insect Biochemistry and Molecular Biology·Jiasheng SongShutang Zhou
Aug 1, 2015·Insect Biochemistry and Molecular Biology·Neil Audsley, Rachel E Down
Feb 27, 2016·Scientific Reports·Alexandre de MendozaMarkus Friedrich
Mar 24, 2016·Insect Molecular Biology·R-Y XiaJ Huang
Oct 24, 2017·Annual Review of Entomology·Sourav RoyAlexander S Raikhel
Jun 12, 2018·Insect Science·Kang LiSheng Li
May 22, 2019·Current Opinion in Insect Science·Carolina Gonçalves SantosKlaus Hartfelder
May 22, 2019·Current Opinion in Insect Science·Cynthia LenaertsJozef Vanden Broeck
Nov 15, 2019·Cellular and Molecular Life Sciences : CMLS·Jiasheng Song, Shutang Zhou

❮ Previous
Next ❯

Citations

Mar 2, 2021·Archives of Insect Biochemistry and Physiology·Junxiu WangShuping Xing
Feb 27, 2021·Frontiers in Cell and Developmental Biology·Zhongxia WuShutang Zhou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.