Identification of GABA(B) receptor in rat testis and sperm

Biochemical and Biophysical Research Communications
X B HeS S Koide

Abstract

gamma-Aminobutyric acid (GABA) can mimic and potentiate the action of progesterone in initiating the acrosome reaction (AR) of mammalian sperm, indicating that sperm contain receptors for GABA. This contention was validated by identifying the receptor (R) subtype, GABA(A)R, in mammalian sperm. In the present study a second subtype, GABA(B)R, was identified in rat testis and sperm. Total RNAs of rat testis and sperm were prepared and used as template to synthesize the respective cDNAs by the RT-PCR method. Two splice variants of the cDNA coding GABA(B)R1 (GABA(B)R1a and GABA(B)R1c) and GABA(B)R2 were identified. Extracts of rat testis, spermatogenic cells and sperm contained two proteins with estimated molecular sizes of 130 and 100 kDa, corresponding to GABA(B)R1a and GABA(B)R1c/lb, respectively, determined by Western blot using polyclonal anti-GABA(B)R1 antibody. By an indirect immunofluorescence technique, GABA(B)R1 was located on the head of rat sperm. The present finding is the first direct demonstration that mammalian sperm contain GABA(B)R.

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Citations

Feb 18, 2004·The Journal of Reproduction and Development·Xiaobing HeS S Koide
Feb 3, 2005·Biological Reviews of the Cambridge Philosophical Society·Stanley Meizel
Jun 1, 2012·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·María Cecilia Gramajo-BühlerGraciela Sánchez-Toranzo
Oct 12, 2004·The International Journal of Biochemistry & Cell Biology·Shi-feng LiYi-ping Li
Mar 25, 2017·Biology of Reproduction·Alfredo Ramírez-RevecoIlona I Concha
Sep 23, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Mitsuko HayashiYoshinori Moriyama
Apr 11, 2002·Cell Research·Jing Hua Hu, Yuan Chang Yan
Jul 23, 2004·Physiological Reviews·Bernhard BettlerMartin Gassmann
Aug 2, 2007·Journal of Cellular Biochemistry·René L VidalAndrés Couve
Jul 31, 2007·Microscopy Research and Technique·Paola RamoinoRenata Manconi
Feb 1, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tiffany J MellottJan Krzysztof Blusztajn
Nov 27, 2009·Systems Biology in Reproductive Medicine·JinFu ZhangLiHe Guo
Dec 10, 2003·Journal of Andrology·Jia Hua HuLi He Guo
Jul 20, 2002·Archives of Biochemistry and Biophysics·Malika BendahmaneDaulat R P Tulsiani

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