Dec 15, 2015

Identification of gallic acid based glycoconjugates as a novel tubulin polymerization inhibitors

Organic & Biomolecular Chemistry
Kapil UpadhyayaRama Pati Tripathi

Abstract

A novel class of gallic acid based glycoconjugates were designed and synthesized as potential anticancer agents. Among all the compounds screened, compound 2a showed potent anticancer activity against breast cancer cells. The latter resulted in tubulin polymerization inhibition and induced G2/M cell cycle arrest, generation of reactive oxygen species, mitochondrial depolarization and subsequent apoptosis in breast cancer cells. In addition, ultraviolet-visible spectroscopy and fluorescence quenching studies of the compound with tubulin confirmed direct interaction of compounds with tubulin. Molecular modeling studies revealed that it binds at the colchicine binding site in tubulin. Further, 2a also exhibited potent in vivo anticancer activity in LA-7 syngeneic rat mammary tumor model. Current data projects its strong candidature to be developed as anticancer agent.

Mentioned in this Paper

Colchicine-Site Binding Agent
Study
In Vivo
Antineoplastic Agents
TUBE1 gene
Apoptosis, Intrinsic Pathway
Inhibition of Protein Polymerization
Structure-Activity Relationship
Molecular Modeling
Isogeneic Graft

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