Identification of GCD14 and GCD15, novel genes required for translational repression of GCN4 mRNA in Saccharomyces cerevisiae.

Genetics
R CuestaM Tamame

Abstract

In Saccharomyces cerevisiae, expression of the transcriptional activator GCN4 increases at the translational level in response to starvation for an amino acid. The products of multiple GCD genes are required for efficient repression of GCN4 mRNA translation under nonstarvation conditions. The majority of the known GCD genes encode subunits of the general translation initiation factor eIF-2 or eIF-2B. To identify additional initiation factors in yeast, we characterized 65 spontaneously arising Gcd- mutants. In addition to the mutations that were complemented by known GCD genes or by GCN3, we isolated mutant alleles of two new genes named GCD14 and GCD15. Recessive mutations in these two genes led to highly unregulated GCN4 expression and to derepressed transcription of genes in the histidine biosynthetic pathway under GCN4 control. The derepression of GCN4 expression in gcd14 and gcd15 mutants occurred with little or no increase in GCN4 mRNA levels, and it was dependent on upstream open reading frames (uORFs) in GCN4 mRNA that regulate its translation. We conclude that GCD14 and GCD15 are required for repression of GCN4 mRNA translation by the uORFs under conditions of amino acid sufficiency. The gcd14 and gcd15 mutations confer...Continue Reading

References

Aug 5, 1975·Journal of Molecular Biology·M WolfnerG R Fink
Jun 1, 1992·Microbiological Reviews·W C Merrick
Jun 1, 1991·Molecular and Cellular Biology·A M CiganA G Hinnebusch
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A M CiganT F Donahue
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·N P WilliamsT F Donahue
Jan 1, 1987·Methods in Enzymology·J D BoekeG R Fink
Apr 25, 1986·Cell·P P Mueller, A G Hinnebusch
Aug 1, 1984·Molecular and Cellular Biology·M Johnston, R W Davis
Jul 1, 1984·Molecular and Cellular Biology·G LucchiniG R Fink
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A G Hinnebusch, G R Fink
Oct 1, 1994·Trends in Biochemical Sciences·A G Hinnebusch
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T E DeverA G Hinnebusch
Jan 1, 1994·Molecular and Cellular Biology·C M Grant, A G Hinnebusch
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A M CiganA G Hinnebusch

❮ Previous
Next ❯

Citations

Sep 13, 2005·Annual Review of Microbiology·Alan G Hinnebusch
Jun 6, 2007·Molecular and Cellular Biology·Pilar Martín-MarcosMercedes Tamame
Jul 25, 1998·The Journal of Biological Chemistry·K J GerikP M Burgers
May 18, 2004·Genes & Development·Sujatha KadabaJames Anderson
Nov 22, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Xiaoting QiuYongxiang Gao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.