Identification of genes expressed during early somatic embryogenesis in Pinus radiata

Plant Physiology and Biochemistry : PPB
Felipe Aquea, Patricio Arce-Johnson

Abstract

Analysis of cDNA-AFLPs was used to study gene expression underlying the early embryogenic process in the gymnosperm Pinus radiata. Somatic embryogenesis in this species was used as a model as it resulted in the generation of a large number of embryos at defined stages of development. The gene expression patterns of three embryogenic stages were compared with non-embryogenic cells. Fifty transcript-derived fragments (TDFs) that are upregulated and 32 TDFs that are down-regulated in the embryogenic stages were selected, sequenced and their homologies sought in the databases. Expression of a selected subset of differentially expressed genes was confirmed by RT-PCR and their levels of expression were quantified. Of the 50 up-regulated TDFs, 16 are homologous to genes encoding either known or putative proteins in higher plants, 19 are homologous to conifer ESTs and 15 did not show significant matches. Of the down-regulated TDFs, 8 are homologous to genes encoding either known or putative proteins, 20 are homologous to conifer ESTs and 4 of them did not show significant matches in DNA or protein sequence database. The known up-regulated genes were similar to genes involved in cellular metabolism and in the stress response and the kno...Continue Reading

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Citations

Aug 5, 2009·Plant Cell Reports·Felipe AqueaPatricio Arce-Johnson
Feb 2, 2010·Journal of Experimental Botany·Felipe AqueaPatricio Arce-Johnson
Dec 2, 2009·BMC Genomics·Kai N StöltingAnthony B Wilson
Mar 14, 2014·Journal of Experimental Botany·Andrei Smertenko, Peter V Bozhkov
Nov 22, 2011·Plant Physiology and Biochemistry : PPB·Anita WiśniewskaMarcin Filipecki
Jun 20, 2018·Physiologia Plantarum·Federica BrunoniCatherine Bellini
Jan 20, 2011·Nature Reviews. Genetics·David B Neale, Antoine Kremer
May 22, 2010·The Protein Journal·Betul CanNazmi Ozer
Dec 31, 2018·BMC Plant Biology·Andreia S RodriguesCélia M Miguel
Aug 1, 2014·Proteome Science·Ellen de Moura ValeVanildo Silveira

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