The extent of gene regulation in cell differentiation is poorly understood. We previously used saturation mutagenesis to identify 18 genes that are needed for the development and function of a single type of sensory neuron--the touch receptor neuron for gentle touch in Caenorhabditis elegans. One of these genes, mec-3, encodes a transcription factor that controls touch receptor differentiation. By culturing and isolating wild-type and mec-3 mutant cells from embryos and applying their amplified RNA to DNA microarrays, here we have identified genes that are known to be expressed in touch receptors, a previously uncloned gene (mec-17) that is needed for maintaining touch receptor differentiation, and more than 50 previously unknown mec-3-dependent genes. These genes are randomly distributed in the genome and under-represented both for genes that are co-expressed in operons and for multiple members of gene families. Using regions 5' of the start codon of the first 20 genes, we have also identified an over-represented heptanucleotide, AATGCAT, that is needed for the expression of touch receptor genes.
The mec-3 gene of Caenorhabditis elegans requires its own product for maintained expression and is expressed in three neuronal cell types
mec-7 is a beta-tubulin gene required for the production of 15-protofilament microtubules in Caenorhabditis elegans
mec-3, a homeobox-containing gene that specifies differentiation of the touch receptor neurons in C. elegans
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Two functionally dependent acetylcholine subunits are encoded in a single Caenorhabditis elegans operon
Genome-wide analysis of developmental and sex-regulated gene expression profiles in Caenorhabditis elegans
Neuron cell type-specific SNAP-25 expression driven by multiple regulatory elements in the nematode Caenorhabditis elegans
LIM homeobox gene-dependent expression of biogenic amine receptors in restricted regions of the C. elegans nervous system
The MEC-4 DEG/ENaC channel of Caenorhabditis elegans touch receptor neurons transduces mechanical signals
Primary culture of Caenorhabditis elegans developing embryo cells for electrophysiological, cell biological and molecular studies
The INTACT method for cell type-specific gene expression and chromatin profiling in Arabidopsis thaliana
The major alpha-tubulin K40 acetyltransferase alphaTAT1 promotes rapid ciliogenesis and efficient mechanosensation
Microtubule depolymerization in Caenorhabditis elegans touch receptor neurons reduces gene expression through a p38 MAPK pathway
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Analysis of C. elegans intestinal gene expression and polyadenylation by fluorescence-activated nuclei sorting and 3'-end-seq
Integrative analysis of C. elegans modENCODE ChIP-seq data sets to infer gene regulatory interactions
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GExplore: a web server for integrated queries of protein domains, gene expression and mutant phenotypes
Classifying genes to the correct Gene Ontology Slim term in Saccharomyces cerevisiae using neighbouring genes with classification learning
Genomic characterization of a repetitive motif strongly associated with developmental genes in Drosophila
Transcriptome profiling of human hepatocytes treated with Aroclor 1254 reveals transcription factor regulatory networks and clusters of regulated genes
Complementary RNA amplification methods enhance microarray identification of transcripts expressed in the C. elegans nervous system
SigWin-detector: a Grid-enabled workflow for discovering enriched windows of genomic features related to DNA sequences
A compendium of Caenorhabditis elegans regulatory transcription factors: a resource for mapping transcription regulatory networks
Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails
Loss of LIN-35, the Caenorhabditis elegans ortholog of the tumor suppressor p105Rb, results in enhanced RNA interference
Cell-specific microarray profiling experiments reveal a comprehensive picture of gene expression in the C. elegans nervous system
Using microarrays to facilitate positional cloning: identification of tomosyn as an inhibitor of neurosecretion
Roles of the developmental regulator unc-62/Homothorax in limiting longevity in Caenorhabditis elegans
Schistosoma mansoni Stomatin like protein-2 is located in the tegument and induces partial protection against challenge infection
mec-15 encodes an F-box protein required for touch receptor neuron mechanosensation, synapse formation and development
The Wnt receptor Ryk reduces neuronal and cell survival capacity by repressing FOXO activity during the early phases of mutant huntingtin pathogenicity
Caenorhabditis elegans reporter fusion genes generated by seamless modification of large genomic DNA clones
The Caenorhabditis elegans lev-8 gene encodes a novel type of nicotinic acetylcholine receptor alpha subunit
Expression and regulation of an FMRFamide-related neuropeptide gene family in Caenorhabditis elegans
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Identification and distribution of a two-pore domain potassium channel in the CNS of Aplysia californica
Time-lapse imaging and cell-specific expression profiling reveal dynamic branching and molecular determinants of a multi-dendritic nociceptor in C. elegans
Caenorhabditis elegans paraoxonase-like proteins control the functional expression of DEG/ENaC mechanosensory proteins
Gene expression profiling studies on Caenorhabditis elegans dystrophin mutants dys-1(cx-35) and dys-1(cx18)
Genetically separable functions of the MEC-17 tubulin acetyltransferase affect microtubule organization
Sensory neuron fates are distinguished by a transcriptional switch that regulates dendrite branch stabilization
MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch receptor neurons through its stomatin-like domain
Genomic cis-regulatory architecture and trans-acting regulators of a single interneuron-specific gene battery in C. elegans
Identification of thermosensory and olfactory neuron-specific genes via expression profiling of single neuron types
Linking asymmetric cell division to the terminal differentiation program of postmitotic neurons in C. elegans
A simple method for gene expression and chromatin profiling of individual cell types within a tissue
Caenorhabditis elegans cyclin D/CDK4 and cyclin E/CDK2 induce distinct cell cycle re-entry programs in differentiated muscle cells
The Developmental Intestinal Regulator ELT-2 Controls p38-Dependent Immune Responses in Adult C. elegans
Genetic analysis of pathways regulated by the von Hippel-Lindau tumor suppressor in Caenorhabditis elegans
Global prediction of tissue-specific gene expression and context-dependent gene networks in Caenorhabditis elegans
Genome-wide transcriptome mapping analysis identifies organ-specific gene expression patterns along human chromosomes
The tubulin repertoire of C. elegans sensory neurons and its context-dependent role in process outgrowth
Cell type-specific transcriptome profiling in C. elegans using the Translating Ribosome Affinity Purification technique
The Caenorhabditis elegans orthologue of mammalian puromycin-sensitive aminopeptidase has roles in embryogenesis and reproduction
Epileptic-like convulsions associated with LIS-1 in the cytoskeletal control of neurotransmitter signaling in Caenorhabditis elegans
Transcriptome analysis of adult Caenorhabditis elegans cells reveals tissue-specific gene and isoform expression
Inhibition of cell fate repressors secures the differentiation of the touch receptor neurons of Caenorhabditis elegans
Repression of an activity-dependent autocrine insulin signal is required for sensory neuron development in C. elegans
Application of machine learning and visualization of heterogeneous datasets to uncover relationships between translation and developmental stage expression of C. elegans mRNAs
Immunodetection of acetylated alpha-tubulin in stony corals: Evidence for the existence of flagella in coral male germ cells
Identification of nonviable genes affecting touch sensitivity in Caenorhabditis elegans using neuronally enhanced feeding RNA interference
HSF-1 displays nuclear stress body formation in multiple tissues in Caenorhabditis elegans upon stress and following the transition to adulthood
Behavioral and molecular response of the insect parasitic nematode Steinernema carpocapsae to cues emitted by a host, the red palm weevil, Rhynchophorus ferrugineus
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