Identification of genes involved in gonadal sex differentiation and the dimorphic expression pattern in Takifugu rubripes gonad at the early stage of sex differentiation

Fish Physiology and Biochemistry
Hongwei YanYusheng Jiang

Abstract

Quantifying the expression of mRNAs in the gonads at the critical stage of molecular sex differentiation stage might help to clarify the regulatory network during early sex differentiation and provide new information on the role of sex-related genes in gonadal function. In this study, transcriptomic analysis of sex-related genes expression profiles in fugu gonads at 60 and 90 days after hatching (dah) was conducted firstly, and a total of 112,504,991 clean reads, encompassing 28.35 Gb of sequences were retrieved. Twenty-three thousand eight hundred ten genes were found to be expressed in juvenile fugu gonads, and we mainly focused on the differentially expressed genes that have the potential to be involved in the gonadal sex differentiation. For 60-dah juveniles, we identified 1014 genes that were upregulated in the ovary and 1570 that were upregulated in the testis. For 90-dah juveniles, we identified 1287 genes that were upregulated in the ovary and 1500 that were upregulated in the testis. The dimorphic expression patterns of 15 genes in gonads at 30 and 40 dah were further investigate using qPCR. Cyp11b and star were expressed at higher levels in XY than in XX, while cyp11a1 and cyp19a1a were expressed at higher levels in X...Continue Reading

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Citations

Jul 16, 2019·Frontiers in Zoology·Virginia PanaraRalf Janssen
Jul 27, 2021·Frontiers in Cell and Developmental Biology·Chen-Wei Hsu, Bon-Chu Chung

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Datasets Mentioned

BETA
SRR5816367

Methods Mentioned

BETA
RNA-seq
PCR
electrophoresis

Software Mentioned

DESeq
BLASTX
Primer Premier
Tophat2
Blast2 GO
Perl script
BLASTn
BLAST
TopGo
R

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