Identification of genes involved in rice seed priming in the early imbibition stage

Plant Biology
J ChengZ Wang

Abstract

Phase II of seed imbibition is a critical process during seed priming. To identify genes involved in rice seed priming, the altered proteins between the dry and imbibed (24 h) seeds were compared using a two-dimensional gel electrophoresis system in this study. Ten significantly changed proteins (fold change ≥ twofold; P < 0.01) were successfully identified, which could be categorised as carbohydrate and protein biosynthesis and metabolism-related, signalling-related, storage and stress-related proteins. A meta-analysis indicated that the highest expression of the identified genes was at the milk and dough stages and in the endosperm tissue. Quantitative real-time PCR analysis showed that there was significant variation in gene expression (except FAD-dependent oxidoreductase) in embryos during seed priming (0-48 h). The expression of genes associated with stress appeared at the early imbibition stage, while those associated with carbohydrate metabolism, protein synthesis and signalling increased at the late imbibition stage. Three identified proteins (glucose-1-phosphate adenylyltransferase large subunit, aminotransferase and prolamin precursor) had similar transcript and protein expression patterns in embryos. Based on phenoty...Continue Reading

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Citations

Dec 8, 2016·Plant Biology·J T M Elzenga, R M Bekker
Jul 6, 2018·Plant, Cell & Environment·Margarete BaierJörn van Buer
Jan 21, 2017·Frontiers in Plant Science·Enshun XuZhoufei Wang
May 1, 2021·International Journal of Molecular Sciences·Hedia TnaniTobias Kretzschmar

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