Identification of genes involved in the susceptibility of Serratia marcescens to polyquaternium-1

The Journal of Antimicrobial Chemotherapy
Caroline E CodlingJean-Yves Maillard

Abstract

Polyquaternium-1 (PQ-1) is a biocide used commercially in a contact lens disinfecting solution, 'Opti-Free Express (Alcon) Multi-Purpose Disinfecting Solution'. The genetic basis for resistance of Serratia marcescens to PQ-1 was investigated using a random transposon-based mutagenesis approach. S. marcescens was subjected to random transposon mutagenesis using a mini-Tn5 Km2 transposon. Mutants with increased susceptibility to PQ-1 were selected and the disrupted genes were identified. Antibiotic susceptibility profiles were also determined for all of the mutants. A wide range of genes were found to be disrupted in the mutants. The most common were genes associated with the cell membranes, or involved in biosynthesis and metabolism. This study shows that random transposon mutagenesis is an effective tool for the elucidation of mechanisms of action and resistance to biocides. The results support our previous findings that PQ-1 is active against the cytoplasmic membrane of S. marcescens.

Citations

Sep 13, 2011·Expert Opinion on Drug Delivery·Maurizio RolandoMalik Y Kahook
Apr 21, 2012·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Dan I Andersson, Diarmaid Hughes
Dec 8, 2005·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Caroline E CodlingA Denver Russell
Apr 21, 2018·Microbiology Spectrum·Jean-Yves Maillard

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