Identification of genes relevant to symbiosis and competitiveness in Sinorhizobium meliloti using signature-tagged mutants

Molecular Plant-microbe Interactions : MPMI
Nataliya PobigayloA Becker

Abstract

Sinorhizobium meliloti enters an endosymbiosis with alfalfa plants through the formation of nitrogen-fixing nodules. In order to identify S. meliloti genes required for symbiosis and competitiveness, a method of signature-tagged mutagenesis was used. Two sets, each consisting of 378 signature-tagged mutants with a known transposon insertion site, were used in an experiment in planta. As a result, 67 mutants showing attenuated symbiotic phenotypes were identified, including most of the exo, fix, and nif mutants in the sets. For 38 mutants in genes previously not described to be involved in competitiveness or symbiosis in S. meliloti, attenuated competitiveness phenotypes were tested individually. A large part of these phenotypes was confirmed. Moreover, additional symbiotic defects were observed for mutants in several novel genes such as infection deficiency phenotypes (ilvI and ilvD2 mutants) or delayed nodulation (pyrE, metA, thiC, thiO, and thiD mutants).

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Citations

Jul 29, 2008·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Yoshikazu ShimodaShusei Sato
Aug 15, 2008·Molecular Plant-microbe Interactions : MPMI·Maria de las Nieves PeltzerDelphine Capela
Dec 18, 2008·Applied and Environmental Microbiology·Arati V Patankar, Juan E González
Apr 20, 2011·Microbes and Environments·Naoganchaolu BorjiginHisayuki Mitsui
Mar 8, 2014·Critical Reviews in Microbiology·Michael Frederick Dunn
Jun 3, 2015·Molecular Plant-microbe Interactions : MPMI·Raphael LedermannHans-Martin Fischer
Aug 22, 2013·Plant Signaling & Behavior·Eliane MeilhocClaude Bruand
Feb 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jon PentermanGraham C Walker
Jun 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Brice SagotGeneviève Alloing
Jan 16, 2016·Environmental Microbiology·George C diCenzoTurlough M Finan
May 13, 2010·Molecular Plant-microbe Interactions : MPMI·Eliane MeilhocClaude Bruand
Sep 12, 2017·Environmental Microbiology·Rafael RivillaJavier Lloret
Mar 18, 2015·Journal of Bacteriology·Melanie J Barnett, Sharon R Long
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Mar 27, 2020·Genes·Ginaini Grazielli Doin de MouraDelphine Capela
Jan 1, 2018·Current Opinion in Plant Biology·Catherine Masson-Boivin, Joel L Sachs

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