Identification of genes whose expression is altered during mitosuppression in livers of ethinyl estradiol-treated female rats

Carcinogenesis
J ChenJames D Yager

Abstract

In this study, our goal was to identify genes whose expression in liver is altered in female F-344 rats during mitosuppression induced by 42 days of ethinyl estradiol (EE) treatment (Yager et al., Carcinogenesis, 15, 2117-2123, 1994). Northern analysis demonstrated that the mRNA levels for transforming growth factor-beta1 (TGF-beta1) and the mannose 6-phosphate/insulin-like growth factor II receptor were significantly increased by EE treatment. Ten cDNA clones representing mRNAs whose expression was increased two- to four-fold in the mitosuppressed livers were identified by differential display. Sequence analysis revealed that one was homologous to the S-24 ribosomal protein and another to mitochondrial ATPase subunit e. The remaining clones showed no homology to known genes in GenBank. However, the expression of clones 15, 16 and 17 was increased in HepG2 cells following treatment with doxorubicin suggesting their induction by oxidative DNA damage. These results suggest that two independent but interrelated signalling pathways, one mediated through transforming growth factor-beta and the other through oxidative DNA damage, may contribute to hepatic mitosuppression caused by EE, perhaps through activation of cyclin-dependent ki...Continue Reading

Citations

Nov 15, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hui TianJunNian Zheng
May 30, 2006·Biochimica Et Biophysica Acta·Roberto Mazzanti, Cecilia Giulivi
Jan 23, 2004·American Journal of Physiology. Endocrinology and Metabolism·Jin Q ChenJames D Yager
Aug 24, 1999·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J C RockettG G Gibson

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