PMID: 9445024Jan 28, 1998Paper

Identification of genetic determinants responsible for the rapid immunosuppressive activity and the low leukemogenic potential of a variant of Friend leukemia virus, FIS-2

Journal of Virology
H Y DaiAre Dalen

Abstract

An immunosuppressive variant of Friend murine leukemia virus (F-MuLV), FIS-2, induces suppression of the primary antibody response against sheep erythrocytes (SRBC) in adult NMRI mice more efficiently than the prototype F-MuLV clone 57 (cl.57). It is, however, less potent than F-MuLV cl.57 in inducing erythroleukemia upon inoculation into newborn NMRI mice. Nucleotide sequence analysis shows a high degree of homology between the two viruses. Single point mutations are scattered over both the gag and the env encoding regions. The most notable mutations are the deletion of one direct repeat and a few single point mutations occurring in the binding sites for cellular transcriptional factors in the FIS-2 long terminal repeat region (LTR). To define the genetic determinants responsible for the pathogenic properties of FIS-2, we constructed six chimeras between FIS-2 and F-MuLV cl.57. Adult mice were infected with the chimeras, and their primary antibody responses against SRBC were investigated. The results showed that the fragment encompassing the FIS-2 env encoding region SU is responsible for the increased immunosuppressive activity in adult mice. A leukemogenicity assay was also performed by infecting newborn mice with the chimer...Continue Reading

References

Feb 9, 1978·Nature·L L LanierP J Wettstein
Jul 1, 1986·The Journal of Experimental Medicine·C A HoltF Lilly
May 1, 1989·Veterinary Immunology and Immunopathology·E SoldainiM Bendinelli
Jan 1, 1985·Advances in Cancer Research·M BendinelliH Friedman
Feb 1, 1986·The Journal of Experimental Medicine·R P MorrisonB Chesebro
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Jan 1, 1980·Contemporary Topics in Immunobiology·M S McGrathI L Weissman
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Aug 1, 1995·Molecular and Cellular Probes·K L BorgA Dalen
Dec 1, 1995·Immunology Today·S HaraguchiN K Day
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·K J Hasenkrug, B Chesebro

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.