Dec 15, 1994

Identification of genetic events involved in early steps of immortalization of mouse fibroblasts

Biochimica Et Biophysica Acta
S C KaulY Mitsui


The spontaneously immortalized early passaged fibroblasts from three different strains of mouse are observed to represent two distinct stages of immortalization. The cells at stage I are characterized by slow growth rate, contact inhibition and requisition of serum factors for their growth and proliferation. Stage II cells are marked by fast, multilayer growth that is independent of serum supplementation in growth medium and by the elevated levels of the two marker proteins, i.e., p53 and p81. The change from cytosolic distribution of mortalin, a senescence inducing protein (J. Biol. Chem. (1993) 268, 6615-6621; 22239-22242) to the perinuclear locale is detected as an early event during cellular immortalization. Furthermore, the distinct stages could be characterized by thermal analysis of intact cells, that to the best of our knowledge is employed for the first time for the analysis of cellular mortal and immortal phenotypes. The study characterizes at least two distinct end points in rodent transformation suggesting that there are multiple routes to immortalization.

Mentioned in this Paper

Heat shock proteins
Heat-Shock Proteins 70
Specimen Type - Fibroblasts
Senescence Function
Cell Division Phases
Hspa9a protein, mouse
Contact Inhibition
Mice, Nude

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