Identification of genetic variations associated with epsilon-poly-lysine biosynthesis in Streptomyces albulus ZPM by genome sequencing

Scientific Reports
Lin WangQingfa Wu

Abstract

The biosynthesis of the antibiotic epsilon-poly-lysine (ε-PL) in Streptomyces albulus is performed by polylysine synthase (pls); however, the regulatory mechanism of this process is still unknown. Here, we first obtained the complete genome sequence of S. albulus ZPM, which consists of 9,784,577 bp and has a GC content of 72.2%. The genome houses 44 gene clusters for secondary metabolite biosynthesis, in which 20 gene clusters are involved in the biosynthesis of polyketides and nonribosomally synthesized peptides. High-throughput sequencing was further performed, and genetic variants were identified from pooled libraries consisting of the 30 highest-yield mutants or 30 lowest-yield mutants. More than 350 genetic variants associated with ε-PL yield have been identified. One hundred sixty-two affected proteins, from important metabolic enzymes to novel transcriptional regulators, were identified as being related to ε-PL synthesis. HrdD, one of the affected genes, is a sigma factor that shows the most sensitive response to pH change and contains a non-synonymous mutation (A132V) in mutant strains with lower ε-PL yields. Electrophoretic mobility shift assays showed that the pls gene is likely regulated by transcriptional activator ...Continue Reading

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Citations

Nov 4, 2016·Bioprocess and Biosystems Engineering·Liang WangZhonggui Mao
Oct 28, 2019·Bioprocess and Biosystems Engineering·Long PanZhong-Gui Mao
Jul 17, 2016·Applied Biochemistry and Biotechnology·Liang WangZhonggui Mao
Feb 1, 2019·Microbiology Resource Announcements·Kazuya Yamanaka, Yoshimitsu Hamano
Jan 10, 2018·Frontiers in Microbiology·Di SunWeijie Liu
Jul 21, 2020·Applied Microbiology and Biotechnology·Jan KormanecDagmar Homerova
Jan 15, 2019·Journal of Agricultural and Food Chemistry·Delei XuHong Xu
Aug 17, 2021·Frontiers in Bioengineering and Biotechnology·Ziyuan WangShiru Jia

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Datasets Mentioned

BETA
CP006871

Methods Mentioned

BETA
whole-genome shotgun sequencing
PCR
electrophoretic mobility shift
DNA-seq
electrophoresis

Software Mentioned

Bowtie2
Blast2GO
Promer
Scan
BLAST30
VarScan2
MUMmer
house
Illumina
Prodigal

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