Identification of genome-wide binding sites of heat shock factor 1, Hsf1, under basal conditions in the human pathogenic yeast, Candida albicans

AMB Express
Remya NairRajendra Prasad

Abstract

The master regulator of thermal stress response, Hsf1, is also an essential determinant for viability and virulence in Candida albicans. Our recent studies highlighted that apart from ubiquitous roles of Hsf1 at higher temperatures, it also has myriad non-heat shock responsive roles essential under iron deprivation and drug defense. Here, we further explored its implications in the normal cellular functioning, by profiling its genome-wide occupancy using chromatin immuno-precipitation coupled to high-density tiling arrays under basal and iron deprived conditions. Hsf1 recruitment profiles revealed that it binds to promoters of 660 genes of varied functions, under both the conditions, however, elicited variability in intensity of binding. For instance, Hsf1 binding was observed on several genes of oxidative and osmotic stress response, cell wall integrity, iron homeostasis, mitochondrial, hyphal and multidrug transporters. Additionally, the present study divulged a novel motif under basal conditions comprising, -GTGn3GTGn3GTG- where, Hsf1 displays strong occupancy at significant number of sites on several promoters distinct from the heat induced motif. Hence, by binding to and regulating major chaperones, stress responsive genes...Continue Reading

References

Oct 11, 2005·Fungal Genetics and Biology : FG & B·Alexander DünklerJürgen Wendland
Jul 18, 2006·Biochimica Et Biophysica Acta·Roland LillUlrich Mühlenhoff
Mar 19, 2013·PLoS Pathogens·Michelle D Leach, Leah E Cowen
Aug 15, 2015·PLoS Genetics·Prashant R DesaiJoachim F Ernst
Nov 28, 2016·Biochimica Et Biophysica Acta. Molecular Cell Research·Remya NairRajendra Prasad

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Citations

May 7, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Ifeanyi Elibe Mba, Emeka Innocent Nweze
Apr 27, 2021·Frontiers in Microbiology·João Henrique Tadini Marilhano FabriIran Malavazi

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Datasets Mentioned

BETA
GSE110889

Methods Mentioned

BETA
protein folding
ChIP
PCR
feature extraction
Fluorescence
pull down

Software Mentioned

Cytoscape
Jalview
Agilent
Microsoft excel
DNA Analytics analytics
Agilent genomic workbench
LOWESS
MEME
- application
Multiple EM for Motif Elucidation ( MEME )

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