Identification of ginsenoside markers from dry purified extract of Panax ginseng by a dereplication approach and UPLC-QTOF/MS analysis

Journal of Pharmaceutical and Biomedical Analysis
Heejung YangSang Hyun Sung

Abstract

A dry purified extract of Panax ginseng (PEG) was prepared using a manufacturing process that includes column chromatography, acid hydrolysis, and an enzyme reaction. During the manufacturing process, the more polar ginsenosides were altered into less polar forms via cleavage of their sugar chains and structural modifications of the aglycones, such as hydroxylation and dehydroxylation. The structural changes of ginsenosides during the intermediate steps from dried ginseng extract (DGE) to PEG were monitored by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectroscopy (UPLC-QTOF/MS). 22 ginsenosides isolated from PEG were used as the reference standards for determining of unknown ginsenosides and further suggesting of the metabolic markers. The elution order of 22 ginsenosides based on the type of aglycones, and the location and number of sugar chains can be used for the structural elucidation of unknown ginsenosides. This information could be used in a dereplication process for quick and efficient identification of ginsenoside derivatives in ginseng preparations. A dereplication approach helped the identification of the metabolic markers in the UPLC-QTOF/MS chromatograms during the convers...Continue Reading

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Nov 8, 2014·Journal of Ginseng Research·Heejung YangSang Hyun Sung

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Citations

May 26, 2017·International Journal of Molecular Sciences·Jae Won LeeDae Young Lee
Mar 25, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mayya RazgonovaKirill Golokhvast
Jan 14, 2021·Journal of Ginseng Research·Yuangui YangZhengtao Wang

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