PMID: 11605972Oct 19, 2001Paper

Identification of GlcNAcylation sites of peptides and alpha-crystallin using Q-TOF mass spectrometry

Journal of the American Society for Mass Spectrometry
Robert J Chalkley, Al Burlingame

Abstract

The addition of a single N-acetylglucosamine residue O-linked to serine and threonine residues of nuclear and cytoplasmic proteins is a widespread modification throughout all eukaryotes. The conventional method for detecting and locating sites of modification is a multi-step radioactivity-based protocol. In this paper we show that using quadrupole time-of-flight (Q-TOF) mass spectrometry, modification sites can be identified at a significantly higher sensitivity than previous approaches. This is the first demonstration that sites of O-GlcNAcylation can be identified directly using mass spectrometry.

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Citations

Feb 22, 2002·Journal of Mass Spectrometry : JMS
Dec 2, 2004·Rapid Communications in Mass Spectrometry : RCM·Jason C Rogalski, Juergen Kast
Dec 4, 2009·Biological Procedures Online·Lianwen ZhangPeng Wang
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Hannes Hahne, Bernhard Kuster
Oct 23, 2013·Analytical Chemistry·Julien Jean Pierre MauryAndre Boon-Hwa Choo
May 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Robert J ChalkleyA L Burlingame
Sep 22, 2009·The Journal of Biological Chemistry·John F Valliere-DouglassAlain Balland
Jun 5, 2012·Molecular & Cellular Proteomics : MCP·Hannes HahneBernhard Kuster
Jul 25, 2012·Molecular & Cellular Proteomics : MCP·Hannes Hahne, Bernhard Kuster
May 31, 2012·Molecular & Cellular Proteomics : MCP·Jonathan C TrinidadAlma L Burlingame
Aug 26, 2010·Molecular & Cellular Proteomics : MCP·Sheng PanTeresa A Brentnall
Jun 10, 2010·European Journal of Mass Spectrometry·Zsuzsa DarulaKatalin F Medzihradszky
Mar 7, 2014·Clinical Proteomics·Junfeng Ma, Gerald W Hart
Sep 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Nelly KhidekelLinda C Hsieh-Wilson
Feb 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Henrik MolinaAkhilesh Pandey
Nov 21, 2014·Bioanalysis·Sheng WangRichard D Smith
May 15, 2007·Nature Chemical Biology·Nelly KhidekelLinda C Hsieh-Wilson
Dec 30, 2014·ACS Chemical Biology·Sophia Doll, Alma L Burlingame
Jan 17, 2012·Proteomics·Alexei MedvedevAlexander Archakov
Jan 18, 2006·Methods in Enzymology·Jasna Peter-Katalinić
Jul 28, 2016·Journal of the American Chemical Society·Peter V RobinsonCarolyn R Bertozzi
Nov 3, 2016·Molecular & Cellular Proteomics : MCP·Jason C MaynardKatalin F Medzihradszky
Apr 12, 2016·Molecular BioSystems·Matthew E GriffinLinda C Hsieh-Wilson
Mar 2, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eun J Kim
Jun 21, 2006·Proteomics·Willy MorelleJean-Claude Michalski
Jun 10, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jan HirschMichael A Matthay
Aug 15, 2018·Frontiers in Endocrinology·Caifei Liu, Jing Li
Nov 15, 2015·The Biochemical Journal·Robert J Chalkley
Jan 28, 2021·Scientific Reports·Navratan BagwanAlicia Lundby
Sep 18, 2020·Molecular & Cellular Proteomics : MCP·Jason Maynard, Robert J Chalkley
Jan 9, 2021·Chemical Reviews·Junfeng MaGerald W Hart
Oct 27, 2020·The Analyst·Chuan Fu, Daan M F van Aalten

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