Identification of glucose-fermenting bacteria in a full-scale enhanced biological phosphorus removal plant by stable isotope probing

Microbiology
Jeppe L NielsenPer H Nielsen

Abstract

Microbiology in wastewater treatment has mainly been focused on problem-causing filamentous bacteria or bacteria directly involved in nitrogen and phosphorus removal, and to a lesser degree on flanking groups, such as hydrolysing and fermenting bacteria. However, these groups constitute important suppliers of readily degradable substrates for the overall processes in the plant. This study aimed to identify glucose-fermenting bacteria in a full-scale enhanced biological phosphorus removal (EBPR) wastewater treatment plant (WWTP), and to determine their abundance in similar WWTPs. Glucose-fermenting micro-organisms were identified by an in situ approach using RNA-based stable isotope probing. Activated sludge was incubated anaerobically with (13)C(6)-labelled glucose, and (13)C-enriched rRNA was subsequently reverse-transcribed and used to construct a 16S rRNA gene clone library. Phylogenetic analysis of the library revealed the presence of two major phylogenetic groups of gram-positive bacteria affiliating with the genera Tetrasphaera, Propionicimonas (Actinobacteria), and Lactococcus and Streptococcus (Firmicutes). Specific oligonucleotide probes were designed for fluorescence in situ hybridization (FISH) to specifically target...Continue Reading

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Citations

Apr 20, 2016·FEMS Microbiology Ecology·Tomonori KindaichiJeppe Lund Nielsen
Jun 9, 2016·Current Opinion in Biotechnology·Tillmann LuedersMike Manefield
Apr 21, 2018·International Journal of Systematic and Evolutionary Microbiology·Guo-Wei ZhouYong-Guan Zhu
Mar 15, 2015·Water Science and Technology : a Journal of the International Association on Water Pollution Research·A Miłobędzka, A Muszyński
Apr 11, 2015·Environmental Microbiology·Christopher E LawsonSteven J Hallam
Dec 21, 2020·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Nancy A ElNakerShadi W Hasan

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