PMID: 616061Jun 1, 1977Paper

Identification of glutamine as a hepatic factor which influence the synthesis of collagen by freshly isolated fibroblasts

Scandinavian Journal of Clinical and Laboratory Investigation
T RönnemaaH Ranta

Abstract

Earlier we have found that 35,000 g supernatants of liver homogenates from both normal and hypercholesterolaemic rats stimulate the synthesis of collagen by freshly isolated fibroblasts. Supernatants from the fatty livers of hypercholesterolaemic rats showed greater stimulation. In the present study we fractionated the 35,000 g supernatants using gel filtration, ion-exchange chromatography, paper electrophoresis and paper chromatography. One of the stimulating factors turned out to be glutamine. However, the concentration of glutamine was the same in normal and fatty livers suggesting that glutamine is not responsible for the greater stimulating activity found in the 35,000 g supernatants from fatty livers. Authentic glutamine increased the synthesis of collagen by 40% at a concentration of 40 mumol/l but inhibited it 80% at 4 mmol/l, which is widely used in cell culture media. There, the concentration of glutamine should be controlled carefully in tests for collagen synthesis in vitro.

References

Nov 1, 1973·The Journal of Clinical Investigation·J T Galambos, R Shapira
Mar 1, 1973·In Vitro·N Blumenkrantz, G Asboe-Hansen
Apr 1, 1966·Analytical Biochemistry·K Juva, D J Prockop
May 1, 1965·The American Journal of Physiology·S SAITO, L C FILLIOS
Jan 1, 1972·Methods in Enzymology·W R Gray

❮ Previous
Next ❯

Citations

Jan 1, 1987·Archives of Dermatological Research·A ChamsonJ Frey
May 1, 1984·Journal of Clinical Pathology·A FallonJ O'D McGee
Nov 1, 1985·Clinical and Experimental Pharmacology & Physiology·A Chamson, J Frey
Sep 21, 1995·Biochimica Et Biophysica Acta·G BellonJ P Borel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.