PMID: 18198479Jan 17, 2008Paper

Identification of glutathione depletion-responsive genes using phorone-treated rat liver

The Journal of Toxicological Sciences
Naoki KiyosawaTetsuro Urushidani

Abstract

To identify candidate biomarker gene sets to evaluate the potential risk of chemical-induced glutathione depletion in livers, we conducted microarray analysis on rat livers administered with phorone (40, 120 and 400 mg/kg), a prototypical glutathione depletor. Hepatic glutathione content was measured and glutathione depletion-responsive gene probe sets (GSH probe sets) were identified using Affymetrix Rat Genome 230 2.0 GeneChip by the following procedure. First, probe sets, whose signal values were inversely correlated with hepatic glutathione content throughout the experimental period, were statistically identified. Next, probe sets, whose average signal values were greater than 1.5-fold compared to those of controls 3 hr after phorone treatment, were selected. Finally, probe sets without unique Entrez Gene ID were removed, ending up with 161 probe sets in total. The usefulness of the identified GSH probe sets was verified by a toxicogenomics database. It was shown that signal profiles of the GSH probe sets in rats treated with bromobenzene were strongly altered compared with other chemicals. Focusing on bromobenzene, time-course profiles of hepatic glutathione content and gene expression revealed that the change in gene expr...Continue Reading

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Citations

Jun 28, 2011·Chemical Research in Toxicology·Yen LowAlexander Tropsha
Mar 1, 2009·Journal of Toxicologic Pathology·Naoki KiyosawaTakashi Yamoto
Oct 20, 2010·International Journal of Molecular Sciences·Naoki KiyosawaAtsushi Sanbuissho
May 19, 2009·Toxicology Letters·Naoki KiyosawaTakashi Yamoto
Jul 19, 2012·Journal of Applied Toxicology : JAT·Fumihiro YamadaYasuo Ohno
Dec 31, 2009·Molecular Nutrition & Food Research·Takeki UeharaTetsuro Urushidani
Aug 7, 2012·The Journal of Toxicological Sciences·Chiaki KondoTakeki Uehara
Jun 2, 2009·The Journal of Toxicological Sciences·Mitsuhiro HirodeTetsuro Urushidani
Feb 28, 2008·The Journal of Toxicological Sciences·Takeki UeharaTetsuro Urushidani
Apr 7, 2010·Genomics·Naoki KiyosawaTakashi Yamoto
Dec 23, 2016·The Journal of Toxicological Sciences·Naoki Kiyosawa, Sunao Manabe
Sep 8, 2009·Human & Experimental Toxicology·M HirodeT Urushidani
Feb 16, 2019·Journal of Applied Toxicology : JAT·Akiko MatsubaraTsuyoshi Yokoi
Mar 31, 2010·Expert Review of Gastroenterology & Hepatology·Qiang ShiWeida Tong
Jul 19, 2017·The Journal of Toxicological Sciences·Noriyuki NakatsuHiroshi Yamada
Dec 18, 2019·International Journal of Toxicology·ChingYi Jennifer HsiehMartha S Sandy
Aug 7, 2012·The Journal of Toxicological Sciences·Nakatsu NoriyukiTetsuro Urushidani
Sep 26, 2020·Annual Review of Pharmacology and Toxicology·Tsuyoshi Yokoi, Shingo Oda

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