Identification of gravitropic response indicator genes in Arabidopsis inflorescence stems

Plant Signaling & Behavior
Masatoshi TaniguchiMiyo Terao Morita

Abstract

Differential organ growth during gravitropic response is caused by differential accumulation of auxin, that is, relative higher auxin concentration in lower flanks than in upper flanks of responding organs. Auxin responsive reporter systems such as DR5::GUS and DR5::GFP have usually been used as indicators of gravitropic response in roots and hypocotyls of Arabidopsis. However, in the inflorescence stems, the reporter systems don't work well to monitor gravitropic response. Here, we aim to certify appropriate gravitropic response indicators (GRIs) in inflorescence stems. We performed microarray analysis comparing gene expression profiles between upper and lower flanks of Arabidopsis inflorescence stems after gravistimulation. Thirty genes showed > 2-fold differentially increased expression in lower flanks at 30 min, of which 19 were auxin response genes. We focused on IAA5 and IAA2 and verified whether they are appropriate GRIs by real-time qRT-PCR analyses. Transcript levels of IAA5 and IAA2 were remarkably higher in lower flanks than in upper flanks after gravistimulation. The biased IAA5 or IAA2 expression is disappeared in sgr2-1 mutant which is defective in gravity perception, indicating that gravity perception process is ...Continue Reading

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Citations

Jan 7, 2016·Frontiers in Plant Science·Oliver SchülerMaik Böhmer
May 20, 2015·Molecular Plant·Hong Ren, William M Gray
Jul 29, 2015·Protoplasma·Yaroslav S KolesnikovVolodymyr S Kravets
Mar 5, 2020·The Plant Journal : for Cell and Molecular Biology·Lucas Cortés LlorcaIan T Baldwin
Jun 25, 2018·Molecular Biology Reports·Nesrine SghaierAhmed Rebai
Jun 3, 2021·Plants·Alexander TkachenkoLudmila Lutova

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Methods Mentioned

BETA
transgenic

Software Mentioned

LightCycler ® 480
DII
VENUS

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