Identification of group A streptococci by direct fluorometry.

Applied Microbiology
M D Moody, C D Webb

Abstract

A simple direct fluorometric method for rapid identification of group A streptococci is described. The method permits the detection of the organism in mixed cultures without the aid of a microscope and is amenable to automated processing of specimens. Experience with the indirect fluorometric method revealed that nontrypsinized cells from a 10-fold dilution of overnight broth cultures could be stained with uniform brilliance with fluorescent antibody (1:15 dilution) and that fluorescent antibody dissociated from such cells at 55 C for 20 min gave serologically specific fluorometric values. With this information, it was possible to develop a simpler fluorometric test which gave results comparable to those obtained by conventional cultural-precipitin grouping techniques. In the direct test described, cultures from throat swabs were incubated overnight, and cells from a 10-fold dilution were stained with specific fluorescent antibody (1:50 dilution) and then rinsed. The stained specimens were transferred to a continuous-filter paper strip (Whatman 3 MM) and read serially in a Turner 110 fluorometer with Corning 5840 and Wratten 2A filters in place. The reagents used required careful standardization and testing to assure that fluor...Continue Reading

References

May 1, 1966·Applied Microbiology·H M HochbergC A Caceres
Dec 1, 1964·Analytical Biochemistry·R M MCKINNEYG W PEARCE
Jul 1, 1963·American Journal of Public Health and the Nation's Health·M D MoodyC C Winter

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Citations

May 1, 1969·Applied Microbiology·C D Webb, M D Moody

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