Identification of GTP-binding proteins in Fasciola hepatica and Schistosoma mansoni by immunoblotting

Molecular and Biochemical Parasitology
J M Mansour, T E Mansour

Abstract

Both the liver fluke Fasciola hepatica and the blood fluke Schistosoma mansoni have GTP-binding proteins which are part of the trans-membrane signalling system. These proteins require GTP in order to interact with the catalytic subunit of adenylate cyclase. The technique of immunoblotting was used in order to distinguish the GTP-binding proteins Gs, Gi, and Go. Immunoblotting was carried out using antisera prepared against peptides deduced from bovine cDNA clones specific for alpha or beta subunits of known G proteins. A 41-kDa Gs alpha has been identified in S. mansoni and a 42.5-kDa Gs alpha in F. hepatica. A 41-kDa Go alpha was found in both parasites. A 36-kDa G beta was identified in both parasites using antiserum made against bovine transducin.

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·K TitaniE H Fischer
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S M MumbyA G Gilman
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H ItohK Suzuki
Nov 1, 1986·Molecular and Biochemical Parasitology·J M Mansour, T E Mansour
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H K FongM I Simon
Jul 1, 1988·Molecular and Biochemical Parasitology·S J Estey, T E Mansour
Nov 1, 1987·Molecular and Biochemical Parasitology·S J Estey, T E Mansour
Jan 1, 1984·Advances in Parasitology·T E Mansour
Oct 30, 1981·Biochemical and Biophysical Research Communications·B H LeichtlingB E Haley
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J K NorthupA G Gilman
Apr 1, 1981·European Journal of Biochemistry·A D NisbetJ E Fothergill

❮ Previous
Next ❯

Citations

Apr 1, 1993·International Journal for Parasitology·J Walker, J Barrett
Oct 17, 2006·Parasitology Today·W Harnett, M M Harnett
Mar 30, 2006·Parasitology·P RibeiroN Patocka
Feb 1, 1992·Experimental Parasitology·P M WiestW D Bowen
Dec 1, 2012·International Journal for Parasitology. Drugs and Drug Resistance·Paul McVeighAaron G Maule
Jul 1, 1997·Experimental Parasitology·K R GrantW Harnett

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.