Identification of H, K, and N-ras point mutations in stage IB cervical carcinoma

Gynecologic Oncology
E C GrendysR Schlegel

Abstract

The ras oncogenes, Harvey (H), Kirsten (K), and neuroblastoma (N), are a family of genes coding for a membrane-associated protein (p21) which possesses inherent guanine triphosphatase (GTPase) activity. Point mutagenesis at codons 12, 13, and 61 has been implicated in ras activation and subsequent cellular transformation. Given the epidemiologic relationship of HPV infection with cervical carcinoma and the tumorigenic interaction of HPV and mutated ras oncogenes, this study was undertaken to identify if mutated ras oncogenes were present in early invasive cervical carcinomas. A combination of polymerase chain reaction (PCR) and dot-blot hybridization was used to determine the frequency and types of ras point mutants occurring in cervical carcinoma. Thirty-three patients with early-stage cervical carcinoma were identified. DNA was extracted from archival tumor samples. ras genes were PCR amplified using flanking primers and hybridized with a series of labeled allele-specific oligonucleotides corresponding to wild-type forms of K12,61, N12,13,61, and H12,61, as well as to all combinations of substitution mutations (7 wild-type, 45 mutants). ras mutations were identified in 24.2% of specimens. The detected mutations in H, K, and N...Continue Reading

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Citations

Aug 25, 2005·The Journal of Investigative Dermatology·Gausal A KhanDhrubajyoti Chakravarti
Feb 27, 2004·Gynecologic Oncology·Ioannis N MammasDemetrios A Spandidos
Oct 13, 2007·Mutation Research·Dhrubajyoti ChakravartiEleanor G Rogan
Oct 25, 2011·The International Journal of Biological Markers·Jelena RadojicicDemetrios A Spandidos
Jul 25, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Lidia Virginia AlonioAngélica Rita Teyssié

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