Abstract
To date, a number of potential biomarkers for lung squamous cell cancer (SCC) have been identified; however, sensitive biomarkers are currently lacking to detect early stage SCC due to low sensitivity and specificity. In the present study, we compared the 7 serum proteomic profiles of 11 SCC patients, 7 chronic obstructive pulmonary disease (COPD) patients and 7 healthy smokers as controls to identify potential serum biomarkers associated with SCC and COPD. Two-dimensional difference gel electrophoresis (2D-DIGE) and mass-spectrometric analysis (MS) using an affinity column revealed two candidate proteins, haptoglobin (HP) and apolipoprotein 4, as biomarkers of SCC, and α-1-antichymotrypsin as a marker of COPD. The iTRAQ technique was also used to identify SCC-specific peptides. HP protein expression was significantly higher in SCC patients than in COPD patients. Furthermore, two HP protein peptides showed significantly higher serum levels in SCC patients than in COPD patients. We established novel polyclonal antibodies for the two HP peptides and subsequently a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantification of these specific peptides in patient and control sera. The sensitivity of detection by ELISA ...Continue Reading
References
Dec 1, 1991·The Journal of Pathology·T NagaredaK Kotoh
Sep 1, 1993·Genomics·W PollerH H Heidtmann
Nov 18, 1998·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·G C Torre
Jun 10, 2000·European Journal of Clinical Investigation·T IshiiY Ouchi
Apr 30, 2002·International Journal of Cancer. Journal International Du Cancer·Tomotaka SobueUNKNOWN JPHC Study Group. Japanese Public Health Center
Aug 15, 2003·Molecular & Cellular Proteomics : MCP·Radhakrishna S TirumalaiTimothy D Veenstra
Sep 8, 2004·Proteomics·Masahiro SeikeSetsuo Hirohashi
Jul 5, 2005·Proteomics·Masahiro SeikeSetsuo Hirohashi
Jun 13, 2006·Proteomics·Tetsuya OkanoSetsuo Hirohashi
Dec 15, 2006·The New England Journal of Medicine·Alan SandlerDavid H Johnson
Feb 10, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Tetsuya OkanoSetsuo Hirohashi
Oct 6, 2007·Cancer·Luke F M HoaglandEdward F Patz
Nov 28, 2007·Electrophoresis·Paul DowlingMartin Clynes
May 29, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Giorgio Vittorio ScagliottiDavid Gandara
Nov 4, 2008·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Martin LatterichBrian Leyland-Jones
Dec 22, 2009·The Lancet Oncology·Tetsuya MitsudomiUNKNOWN West Japan Oncology Group
Mar 23, 2010·Respiratory Medicine·Emer KellyShane J O'Neill
Jun 25, 2010·The New England Journal of Medicine·Makoto MaemondoUNKNOWN North-East Japan Study Group
Oct 19, 2011·Cancer·Yuri TakedaEiji Miyoshi
Feb 14, 2012·Circulation Journal : Official Journal of the Japanese Circulation Society·UNKNOWN JCS Joint Working GroupUNKNOWN Japan Lung Cancer Society
Dec 1, 2012·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Helen A PowellLaila J Tata
Jan 22, 2013·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Jul 19, 2013·The New England Journal of Medicine·Stephanie A KovalchikHormuzd A Katki
Dec 4, 2014·The New England Journal of Medicine·Benjamin J SolomonUNKNOWN PROFILE 1014 Investigators
Dec 19, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Bing WangHong Qiu
Citations
Aug 3, 2019·Cells·Ángela MarrugalLuis Paz-Ares
Jul 28, 2017·PloS One·Zhen-Ming TangJin-Liang Kong
Oct 18, 2019·PloS One·Andrzej CiereszkoJacek Nikliński
Oct 27, 2017·Oncotarget·Jian XiaoQiong Chen
May 18, 2020·Proteomics. Clinical Applications·Roberto GasparriLorenzo Spaggiari
Dec 21, 2016·PloS One·Fenming ZhangGuoqiang Xu
Mar 13, 2019·Scientific Reports·Mahadi HasanKentaro Kogure
Aug 10, 2019·Cell Death Discovery·Bin YangYiyu Yin
Nov 10, 2017·Mass Spectrometry Reviews·Robert MoulderRiitta Lahesmaa
Jan 2, 2019·Clinical Proteomics·Jaimie DufresneJohn G Marshall
Mar 30, 2019·Journal of Occupational Medicine and Toxicology·Simone HelmigJoachim Schneider
Nov 22, 2020·Journal of Clinical Laboratory Analysis·Yang XiaoZhen Jiang
Jul 15, 2020·Molecular Aspects of Medicine·Alessandra di MasiPaolo Ascenzi
Apr 17, 2021·Biomedit︠s︡inskai︠a︡ khimii︠a︡·S N Naryzhny, O K Legina
Aug 24, 2021·Biochemistry (Moscow) Supplement. Series B, Biomedical Chemistry·S N Naryzny, O K Legina