Identification of haptoglobin peptide as a novel serum biomarker for lung squamous cell carcinoma by serum proteome and peptidome profiling

International Journal of Oncology
Tetsuya OkanoAkihiko Gemma

Abstract

To date, a number of potential biomarkers for lung squamous cell cancer (SCC) have been identified; however, sensitive biomarkers are currently lacking to detect early stage SCC due to low sensitivity and specificity. In the present study, we compared the 7 serum proteomic profiles of 11 SCC patients, 7 chronic obstructive pulmonary disease (COPD) patients and 7 healthy smokers as controls to identify potential serum biomarkers associated with SCC and COPD. Two-dimensional difference gel electrophoresis (2D-DIGE) and mass-spectrometric analysis (MS) using an affinity column revealed two candidate proteins, haptoglobin (HP) and apolipoprotein 4, as biomarkers of SCC, and α-1-antichymotrypsin as a marker of COPD. The iTRAQ technique was also used to identify SCC-specific peptides. HP protein expression was significantly higher in SCC patients than in COPD patients. Furthermore, two HP protein peptides showed significantly higher serum levels in SCC patients than in COPD patients. We established novel polyclonal antibodies for the two HP peptides and subsequently a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantification of these specific peptides in patient and control sera. The sensitivity of detection by ELISA ...Continue Reading

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Citations

May 18, 2020·Proteomics. Clinical Applications·Roberto GasparriLorenzo Spaggiari
Aug 10, 2019·Cell Death Discovery·Bin YangYiyu Yin
Nov 10, 2017·Mass Spectrometry Reviews·Robert MoulderRiitta Lahesmaa
Jan 2, 2019·Clinical Proteomics·Jaimie DufresneJohn G Marshall
Mar 30, 2019·Journal of Occupational Medicine and Toxicology·Simone HelmigJoachim Schneider
Jul 15, 2020·Molecular Aspects of Medicine·Alessandra di MasiPaolo Ascenzi
Apr 17, 2021·Biomedit︠s︡inskai︠a︡ khimii︠a︡·S N Naryzhny, O K Legina
Aug 24, 2021·Biochemistry (Moscow) Supplement. Series B, Biomedical Chemistry·S N Naryzny, O K Legina

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Methods Mentioned

BETA
X-ray
electrophoresis
enzyme-linked immunosorbent assay
ELISA
PCA
glycosylation

Software Mentioned

ProteinPilot
Impressionist
Excel
DeCyder
Analyst QS
Mascot

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