Identification of hematopoietic stem/progenitor cells: strength and drawbacks of functional assays

Oncogene
L Coulombel

Abstract

A major challenge in hematopoiesis is to conceive assays that could bring useful insights into experimental and clinical hematology. This means identifying separately the various classes of hematopoietic progenitors that are produced sequentially during the progression from stem cells to differentiated functional cells. Standardized short-term colony assays easily quantify lineage-committed myeloid precursors, but identification of primitive cells, which have both the ability to repopulate durably myeloid and lymphoid lineages and perhaps to self-renew, still depends on in vivo assays. Whatever the assay, two important requisites have to be solved: one is the definition of appropriate read-outs that will depend solely on the function of these cells, and the second is to evaluate precisely their numbers and proliferative potential in quantitative assays. When evaluating hematopoiesis, three parameters have to be taken into account: (1) the lack of reliable correlation between the phenotype of a given cell and its function. This is especially problematic in post-transplantation situations where cells from transplanted animals are analysed; (2) functionally heterogeneous cells are identified in a single assay; and (3) ontogeny-rel...Continue Reading

References

Feb 15, 1975·Lancet·J StothersG Snodgrass
Jun 1, 1977·Journal of Cellular Physiology·T M DexterL G Lajtha
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C M BaumB Peault
Feb 1, 1990·Genes & Development·C T Jordan, I R Lemischka
May 1, 1990·The Journal of Experimental Medicine·G Keller, R Snodgrass
Oct 1, 1990·European Journal of Immunology·A CumanoC J Paige
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S J SzilvassyC J Eaves
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H J SutherlandC J Eaves
Jan 1, 1984·Critical Reviews in Oncology/hematology·H E Broxmeyer
Mar 16, 1984·Journal of Immunological Methods·C A WhitlockO N Witte
Feb 11, 1982·Nature·M C MagliN Odartchenko
Jul 1, 1994·The Journal of Experimental Medicine·A BárcenaH Spits
Sep 1, 1993·The Journal of Experimental Medicine·P M LansdorpH Mayani
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·I GodinA Cumano
Mar 1, 1994·The Journal of Clinical Investigation·E D ZanjaniM Tavassoli
Apr 1, 1994·Current Opinion in Immunology·J ChenF W Alt
Jan 1, 1996·Annual Review of Immunology·G AndersonE J Jenkinson
Jan 1, 1996·International Reviews of Immunology·E Spanopoulou
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·P W ZandstraC J Eaves
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·M BhatiaJ E Dick

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Citations

Feb 22, 2012·Nature Cell Biology·Cristina PinaTariq Enver
Nov 14, 2008·Stem Cells and Development·Cristina RofaniAnna C Berardi
Mar 24, 2011·International Immunology·Yana Khalfin-RabinovichBen-Zion Levi
Jun 10, 2008·Current Opinion in Hematology·Mariusz Z Ratajczak
Oct 1, 2009·Journal of Gastroenterology and Hepatology·Mélanie A Eckersley-MaslinNicholas A Shackel
Jan 22, 2011·Journal of Visualized Experiments : JoVE·Nayan J SarmaNabeel R Yaseen
May 19, 2012·Regenerative Medicine·Thomas Clapes, Catherine Robin
Nov 28, 2012·British Medical Bulletin·Caterina PipinoPaolo De Coppi
Jun 13, 2009·Cell Transplantation·Xiaosong HuangGerald J Spangrude
Mar 22, 2005·Bone Marrow Transplantation·J J Auletta, H M Lazarus
Feb 10, 2016·Pflügers Archiv : European journal of physiology·Inês Alvarez-MartinsSérgio Dias
Sep 4, 2007·Gynécologie, obstétrique & fertilité·L Coulombel
Jul 17, 2008·Biological Chemistry·Bernd Giebel, Michael Punzel
Jul 27, 2005·British Journal of Haematology·Greg R AngstreichRichard J Jones
Feb 4, 2006·Stem Cells·Leonie M Kamminga, Gerald de Haan
Apr 16, 2008·British Journal of Haematology·Michelle ChuDarryl Shibata
Jul 30, 2009·Molecular Systems Biology·Daniel C KirouacPeter W Zandstra
Nov 19, 2011·Oral Diseases·A LeventhalM Boehm
Sep 17, 2010·Stem Cells·Sandrine PoglioBéatrice Cousin
Feb 26, 2013·Respirology : Official Journal of the Asian Pacific Society of Respirology·Ivan Bertoncello, Jonathan L McQualter
Aug 11, 2006·Current Opinion in Biotechnology·Daniel C Kirouac, Peter W Zandstra
Aug 8, 2006·Current Opinion in Biotechnology·Won Jae HuhJason C Mills
Jul 31, 2012·Current Opinion in Immunology·Katja G WeinachtLuigi D Notarangelo
May 15, 2015·Experimental Hematology·Nicolas Pineault, Ahmad Abu-Khader
May 21, 2008·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Kirsten E HerbertMiles Prince
Mar 25, 2014·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Meltem ÖzgünerBahattin Tunç
Apr 29, 2016·Nature Protocols·Ondrej SvobodaPetr Bartunek

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