PMID: 8595867Jan 15, 1996Paper

Identification of heme-binding proteins in the cell membranes of Vibrio anguillarum

FEMS Microbiology Letters
Ramón Mazoy, Manuel L Lemos

Abstract

Two strains of Vibrio anguillarum belonging to O1 and O2 serotypes were examined for their ability to bind hemin and hemoglobin. Whole cells as well as membrane extracts from both strains could clearly bind hemin and hemoglobin constitutively. Hemoglobin binding was completely inhibited by a 100-fold excess of unlabelled hemoglobin and also by hemin, suggesting the existence of specific receptors for heme groups in the cell membranes. Several hemin-binding and hemoglobin-binding bands with similar molecular sizes were detected in polyacrylamide gels as well as in Western blots. Only two of these protein bands in both strains were iron-regulated while the others were independent of the cell iron status. We conclude that both serotypes of V. anguillarum possess heme-binding abilities by means of membrane proteins.

References

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Jan 1, 1992·Critical Reviews in Microbiology·B R OttoD M MacLaren
Apr 1, 1988·Journal of Bacteriology·M L LemosJ H Crosa
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May 15, 1994·FEMS Microbiology Letters·M E FrangipaneT L Stull

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Citations

Sep 3, 2004·Journal of Bacteriology·Susana MouriñoManuel L Lemos
Nov 5, 2005·Applied and Environmental Microbiology·Ruben Avendaño-HerreraBeatriz Magariños
Aug 10, 2017·Frontiers in Cellular and Infection Microbiology·Yingjie Li, Qingjun Ma
Dec 15, 2015·Microbiology and Molecular Biology Reviews : MMBR·Shelley M PayneElizabeth E Wyckoff
Mar 1, 1997·FEMS Microbiology Letters·S MiyoshiS Shinoda
Jan 9, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Manuel L Lemos, Carlos R Osorio
Aug 1, 1996·Applied and Environmental Microbiology·B FouzC Amaro

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