Identification of hemolytic granules isolated from human myocardial cells

Experientia
Y KawamotoA Miyama

Abstract

Human myocardial cells from fresh autopsy material contained granules which possessed hemolytic activity against guinea pig and rabbit erythrocytes. The hemolytic granules, which had a density of 1.02 and a diameter of 200-300 nm, were recovered as a microsome fraction from subcellular homogenates of human myocardial cells by differential centrifugation in 300 mM sucrose containing 0.1 mM PMSF and 10 mM EDTA. The membrane lesions caused by the granules were ring-like structures with an internal diameter of about 10-17 nm, analogous to that caused by perforin- and complement-induced lysis. However, the requirement for divalent cation differed from that for perforin-induced lysis, since the microsome-mediated lysis occurred in the presence of EDTA.

References

Jan 1, 1986·Journal of Cellular Biochemistry·E R Podack
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L S ZalmanH J Müller-Eberhard
May 1, 1988·Immunology Today·J D Young, C C Liu
Apr 1, 1985·Nature·M S Pasternack, H N Eisen
Sep 1, 1984·The Journal of Experimental Medicine·E R Podack, P J Konigsberg
Jan 1, 1984·Microbiology and Immunology·K OkamotoA Miyama

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