Identification of hepatitis C virus genotype 6 in Korean patients by analysis of 5' untranslated region using a matrix assisted laser desorption/ionization time of flight-based assay, restriction fragment mass polymorphism

Journal of Medical Virology
Heung-Bum OhDong Jin Suh

Abstract

Previous surveys of the prevalence of hepatitis C virus (HCV) in Korea have identified types 1 and 2, but little has been said of other genotypes and viral subtypes. In this study, HCV genotypes in Korea were investigated using Restriction Fragment Mass Polymorphism (RFMP) assay, a sensitive and specific method for genotyping based on MALDI-TOF mass spectrometry. A total of 1,043 independent serum samples from HCV-infected patients were analyzed. Of interest, 15 subjects (1.4%) were determined to contain HCV genotype 6 and 46 subjects (4.4%) contained mixed genotypes with the most prevalent genotypes being HCV 1b and 2a/c (45.0% and 35.4%, respectively). The 15 subjects with HCV genotype 6 comprised eight cases of subtype 6c, including one case of mixed infection with 1b, three cases of HCV 6a, and six cases of unassigned subtypes. Sequencing corroborated the identity of genotype 6 from 13 subjects, while the line probe assay (LiPA) mis-identified them as genotype 1b. The majority (7/9) of the genotype 6 patients enrolled for interferon/ribavirin therapy, achieved a sustained virologic response. The ability of the RFMP assay to differentiate various HCV genotypes should enable better analysis of the relationship between HCV gen...Continue Reading

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Citations

May 11, 2011·Mass Spectrometry Reviews·Yen-Peng Ho, P Muralidhar Reddy
Mar 14, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J-H LeeS-O Kim
Mar 20, 2015·World Journal of Clinical Cases·Vasilios Papastergiou, Stylianos Karatapanis
Feb 27, 2015·The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi·Seung Kak ShinJu Hyun Kim
Apr 7, 2010·The Korean Journal of Hepatology·Young Suk Lim
Nov 14, 2013·The Open Virology Journal·Fernando Cobo
Feb 20, 2010·Clinical Chemistry·Yen-Peng Ho, P Muralidhar Reddy
Jan 29, 2019·Alimentary Pharmacology & Therapeutics·Panita MettikanontK Rajender Reddy

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